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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Alkaliphilus oremlandii Glycine cleavage system H protein (gcvH) CSB-YP009335AUQ
CSB-EP009335AUQ
CSB-BP009335AUQ
CSB-MP009335AUQ
CSB-EP009335AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Translation initiation factor IF-2 (infB), partial CSB-YP015164AUQ
CSB-EP015164AUQ
CSB-BP015164AUQ
CSB-MP015164AUQ
CSB-EP015164AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii tRNA dimethylallyltransferase (miaA) CSB-YP024529AUQ
CSB-EP024529AUQ
CSB-BP024529AUQ
CSB-MP024529AUQ
CSB-EP024529AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Peptide chain release factor 1 (prfA) CSB-YP015197AUQ
CSB-EP015197AUQ
CSB-BP015197AUQ
CSB-MP015197AUQ
CSB-EP015197AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Elongation factor Tu (tuf1) CSB-YP025342AUQ
CSB-EP025342AUQ
CSB-BP025342AUQ
CSB-MP025342AUQ
CSB-EP025342AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus oremlandii Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917AUQ
CSB-EP022917AUQ
CSB-BP022917AUQ
CSB-MP022917AUQ
CSB-EP022917AUQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit G (Bm1_08615) CSB-YP007536BWV
CSB-EP007536BWV
CSB-BP007536BWV
CSB-MP007536BWV
CSB-EP007536BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Galectin-2 (Cgl2) CSB-YP012886DWQ
CSB-EP012886DWQ
CSB-BP012886DWQ
CSB-MP012886DWQ
CSB-EP012886DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea U1 small nuclear ribonucleoprotein C (CC1G_01380) CSB-YP022331DWQ
CSB-EP022331DWQ
CSB-BP022331DWQ
CSB-MP022331DWQ
CSB-EP022331DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit L (Bm1_25770), partial CSB-YP007541BWV
CSB-EP007541BWV
CSB-BP007541BWV
CSB-MP007541BWV
CSB-EP007541BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malassezia globosa Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) CSB-YP002426MNH
CSB-EP002426MNH
CSB-BP002426MNH
CSB-MP002426MNH
CSB-EP002426MNH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi 40S ribosomal protein SA (Bm1_41245) CSB-YP020485BWV
CSB-EP020485BWV
CSB-BP020485BWV
CSB-MP020485BWV
CSB-EP020485BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi tRNA-splicing ligase RtcB homolog (Bm1_49220), partial CSB-YP003810BWV
CSB-EP003810BWV
CSB-BP003810BWV
CSB-MP003810BWV
CSB-EP003810BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi Eukaryotic translation initiation factor 3 subunit K (Bm1_52955) CSB-YP007540BWV
CSB-EP007540BWV
CSB-BP007540BWV
CSB-MP007540BWV
CSB-EP007540BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brugia malayi DDRGK domain-containing protein 1 (Bm1_54325) CSB-YP006597BWV
CSB-BP006597BWV
CSB-MP006597BWV
CSB-EP006597BWV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Actin-related protein 8 (ARP8) CSB-YP001254OFF
CSB-EP001254OFF
CSB-BP001254OFF
CSB-MP001254OFF
CSB-EP001254OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Probable 3-hydroxymethyl-3-methylglutaryl-CoA lyase 2 (hmgcll1) CSB-YP010564DIL
CSB-EP010564DIL
CSB-BP010564DIL
CSB-MP010564DIL
CSB-EP010564DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Cytosolic Fe-S cluster assembly factor narfl (narfl) CSB-YP015455XBF
CSB-EP015455XBF
CSB-BP015455XBF
CSB-MP015455XBF
CSB-EP015455XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig CD302 antigen (CD302), partial CSB-YP004923PI
CSB-EP004923PI
CSB-BP004923PI
CSB-MP004923PI
CSB-EP004923PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytoplasmic tRNA 2-thiolation protein 1 (ctu-1) CSB-YP002426CXX
CSB-EP002426CXX
CSB-BP002426CXX
CSB-MP002426CXX
CSB-EP002426CXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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