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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Salmonella paratyphi B 3-ketoacyl-CoA thiolase (fadI) CSB-YP001117STF
CSB-EP001117STF
CSB-BP001117STF
CSB-MP001117STF
CSB-EP001117STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B N-acetyl-D-glucosamine kinase (nagK) CSB-YP015415STF
CSB-EP015415STF
CSB-BP015415STF
CSB-MP015415STF
CSB-EP015415STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Translation initiation factor IF-2 (infB), partial CSB-YP015164STF
CSB-EP015164STF
CSB-BP015164STF
CSB-MP015164STF
CSB-EP015164STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Peptide deformylase (def) CSB-YP017707STF
CSB-EP017707STF
CSB-BP017707STF
CSB-MP017707STF
CSB-EP017707STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella paratyphi B Methionyl-tRNA formyltransferase (fmt) CSB-YP015150STF
CSB-EP015150STF
CSB-BP015150STF
CSB-MP015150STF
CSB-EP015150STF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii GTPase Era (era) CSB-YP007759CXH
CSB-EP007759CXH
CSB-BP007759CXH
CSB-MP007759CXH
CSB-EP007759CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii Methionyl-tRNA formyltransferase (fmt) CSB-YP015150CXH
CSB-EP015150CXH
CSB-BP015150CXH
CSB-MP015150CXH
CSB-EP015150CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coxiella burnetii ATP synthase subunit delta (atpH) CSB-YP002355CXH
CSB-EP002355CXH
CSB-BP002355CXH
CSB-MP002355CXH
CSB-EP002355CXH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acholeplasma laidlawii Thymidylate synthase (thyA) CSB-YP025393ATX
CSB-EP025393ATX
CSB-BP025393ATX
CSB-MP025393ATX
CSB-EP025393ATX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033YAM
CSB-EP018033YAM
CSB-BP018033YAM
CSB-MP018033YAM
CSB-EP018033YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799YAM
CSB-EP014799YAM
CSB-BP014799YAM
CSB-MP014799YAM
CSB-EP014799YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Ribosome-recycling factor (frr) CSB-YP014941YAM
CSB-EP014941YAM
CSB-BP014941YAM
CSB-MP014941YAM
CSB-EP014941YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis bv. Antiqua Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717YAM
CSB-EP009717YAM
CSB-BP009717YAM
CSB-MP009717YAM
CSB-EP009717YAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii NADH-ubiquinone oxidoreductase chain 1 (ND1), partial CSB-YP015076DIS
CSB-EP015076DIS
CSB-BP015076DIS
CSB-MP015076DIS
CSB-EP015076DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP synthase protein 8 (ATP8), partial CSB-YP015071DIS
CSB-EP015071DIS
CSB-BP015071DIS
CSB-MP015071DIS
CSB-EP015071DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Intraflagellar transport protein 57 homolog (ift57) CSB-YP011076XBE
CSB-EP011076XBE
CSB-BP011076XBE
CSB-MP011076XBE
CSB-EP011076XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis LYR motif-containing protein 1 (lyrm1) CSB-YP013272XBE
CSB-EP013272XBE
CSB-BP013272XBE
CSB-MP013272XBE
CSB-EP013272XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Pescadillo homolog (19511), partial CSB-YP017791MOD
CSB-EP017791MOD
CSB-BP017791MOD
CSB-MP017791MOD
CSB-EP017791MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis tRNA-splicing ligase RtcB homolog (24995) CSB-YP003810MOD
CSB-EP003810MOD
CSB-BP003810MOD
CSB-MP003810MOD
CSB-EP003810MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monosiga brevicollis Probable methylthioribulose-1-phosphate dehydratase (29994) CSB-YP001906MOD
CSB-EP001906MOD
CSB-BP001906MOD
CSB-MP001906MOD
CSB-EP001906MOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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