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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Spermophilus beecheyi Hemoglobin subunit alpha CSB-YP010147FJZ
CSB-EP010147FJZ
CSB-BP010147FJZ
CSB-MP010147FJZ
CSB-EP010147FJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peromyscus crinitus Hemoglobin subunit beta CSB-YP010150ETV
CSB-EP010150ETV
CSB-BP010150ETV
CSB-MP010150ETV
CSB-EP010150ETV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Microtus pennsylvanicus Hemoglobin subunit alpha CSB-YP010147MTW
CSB-EP010147MTW
CSB-BP010147MTW
CSB-MP010147MTW
CSB-EP010147MTW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146AXI
CSB-EP019146AXI
CSB-BP019146AXI
CSB-MP019146AXI
CSB-EP019146AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 6-phosphofructokinase (pfkA) CSB-YP017822AXI
CSB-EP017822AXI
CSB-BP017822AXI
CSB-MP017822AXI
CSB-EP017822AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Methionyl-tRNA formyltransferase (fmt) CSB-YP015150AXI
CSB-EP015150AXI
CSB-BP015150AXI
CSB-MP015150AXI
CSB-EP015150AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Malate dehydrogenase (mdh) CSB-YP013623AXI
CSB-EP013623AXI
CSB-BP013623AXI
CSB-MP013623AXI
CSB-EP013623AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 ATP synthase subunit a (atpB), partial CSB-YP015070AXI1
CSB-EP015070AXI1
CSB-BP015070AXI1
CSB-MP015070AXI1
CSB-EP015070AXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Plasmodium knowlesi 40S ribosomal protein S3a (PKH_081740) CSB-YP020444EWU
CSB-EP020444EWU
CSB-BP020444EWU
CSB-MP020444EWU
CSB-EP020444EWU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit A (eIF3-S10), partial CSB-YP007543DKY
CSB-EP007543DKY
CSB-BP007543DKY
CSB-MP007543DKY
CSB-EP007543DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Serine/threonine-protein kinase PLK4 (SAK), partial CSB-YP018196DKY
CSB-EP018196DKY
CSB-BP018196DKY
CSB-MP018196DKY
CSB-EP018196DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Protein N-terminal glutamine amidohydrolase (tun) CSB-YP026083DKY
CSB-EP026083DKY
CSB-BP026083DKY
CSB-MP026083DKY
CSB-EP026083DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DKY
CSB-EP007530DKY
CSB-BP007530DKY
CSB-MP007530DKY
CSB-EP007530DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Probable methylthioribulose-1-phosphate dehydratase (GF22600) CSB-YP001906DKY
CSB-EP001906DKY
CSB-BP001906DKY
CSB-MP001906DKY
CSB-EP001906DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit H (eIF-3p40) CSB-YP007537DLK
CSB-EP007537DLK
CSB-BP007537DLK
CSB-MP007537DLK
CSB-EP007537DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta 40S ribosomal protein S21 (RpS21) CSB-YP020399DLK
CSB-EP020399DLK
CSB-BP020399DLK
CSB-MP020399DLK
CSB-EP020399DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Serine/threonine-protein kinase PLK4 (SAK), partial CSB-YP018196DLK
CSB-EP018196DLK
CSB-BP018196DLK
CSB-MP018196DLK
CSB-EP018196DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Eukaryotic translation initiation factor 3 subunit C (eIF3-S8), partial CSB-YP007531DLK
CSB-EP007531DLK
CSB-BP007531DLK
CSB-MP007531DLK
CSB-EP007531DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Lysine-specific demethylase NO66 (GG18702), partial CSB-YP003168DLK
CSB-EP003168DLK
CSB-BP003168DLK
CSB-MP003168DLK
CSB-EP003168DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila erecta Ubiquitin-conjugating enzyme E2 S (GG18165) CSB-YP025477DLK
CSB-EP025477DLK
CSB-BP025477DLK
CSB-MP025477DLK
CSB-EP025477DLK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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