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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Drosophila virilis Molybdopterin synthase catalytic subunit (GJ23609) CSB-YP014706DMP
CSB-EP014706DMP
CSB-BP014706DMP
CSB-MP014706DMP
CSB-EP014706DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit K (GK10740) CSB-YP007540DMQ
CSB-EP007540DMQ
CSB-BP007540DMQ
CSB-MP007540DMQ
CSB-EP007540DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit J (Adam) CSB-YP007539DMQ
CSB-EP007539DMQ
CSB-BP007539DMQ
CSB-MP007539DMQ
CSB-EP007539DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein SMG8 (GK15451), partial CSB-YP003375DMQ
CSB-EP003375DMQ
CSB-BP003375DMQ
CSB-MP003375DMQ
CSB-EP003375DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit M (Tango7) CSB-YP007542DMQ
CSB-EP007542DMQ
CSB-BP007542DMQ
CSB-MP007542DMQ
CSB-EP007542DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Eukaryotic translation initiation factor 3 subunit H (eIF-3p40) CSB-YP007537DMQ
CSB-EP007537DMQ
CSB-BP007537DMQ
CSB-MP007537DMQ
CSB-EP007537DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Protein kibra (Kibra), partial CSB-YP026163DMQ
CSB-EP026163DMQ
CSB-BP026163DMQ
CSB-MP026163DMQ
CSB-EP026163DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit J (Adam) CSB-YP007539DMR
CSB-EP007539DMR
CSB-BP007539DMR
CSB-MP007539DMR
CSB-EP007539DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Zinc finger CCCH-type with G patch domain-containing protein (GE18884), partial CSB-YP026480DMR
CSB-EP026480DMR
CSB-BP026480DMR
CSB-MP026480DMR
CSB-EP026480DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein SMG8 (GE13372), partial CSB-YP003375DMR
CSB-EP003375DMR
CSB-BP003375DMR
CSB-MP003375DMR
CSB-EP003375DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Eukaryotic translation initiation factor 3 subunit C (eIF3-S8), partial CSB-YP007531DMR
CSB-EP007531DMR
CSB-BP007531DMR
CSB-MP007531DMR
CSB-EP007531DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Protein arginine N-methyltransferase 7 (Art7), partial CSB-YP018736DMR
CSB-EP018736DMR
CSB-BP018736DMR
CSB-MP018736DMR
CSB-EP018736DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba 40S ribosomal protein SA (sta) CSB-YP020485DMR
CSB-EP020485DMR
CSB-BP020485DMR
CSB-MP020485DMR
CSB-EP020485DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Ubiquitin-conjugating enzyme E2 S (GE15576) CSB-YP025477DMR
CSB-EP025477DMR
CSB-BP025477DMR
CSB-MP025477DMR
CSB-EP025477DMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Protein SMG8 (GD22229), partial CSB-YP003375DMJ
CSB-EP003375DMJ
CSB-BP003375DMJ
CSB-MP003375DMJ
CSB-EP003375DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Eukaryotic translation initiation factor 3 subunit B (eIF3-S9), partial CSB-YP007530DMJ
CSB-EP007530DMJ
CSB-BP007530DMJ
CSB-MP007530DMJ
CSB-EP007530DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Eukaryotic translation initiation factor 3 subunit L (GD12745), partial CSB-YP007541DMJ
CSB-EP007541DMJ
CSB-BP007541DMJ
CSB-MP007541DMJ
CSB-EP007541DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Methylthioribose-1-phosphate isomerase (GD16414) CSB-YP014799DMJ
CSB-EP014799DMJ
CSB-BP014799DMJ
CSB-MP014799DMJ
CSB-EP014799DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila simulans Nuclear cap-binding protein subunit 2 (Cbp20) CSB-YP015521DMJ
CSB-EP015521DMJ
CSB-BP015521DMJ
CSB-MP015521DMJ
CSB-EP015521DMJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phenylobacterium zucineum Elongation factor G (fusA), partial CSB-YP009373EUP
CSB-EP009373EUP
CSB-BP009373EUP
CSB-MP009373EUP
CSB-EP009373EUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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