Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Cyanothece sp. Adenosylhomocysteinase (ahcY) CSB-YP001474DZZ
CSB-EP001474DZZ
CSB-BP001474DZZ
CSB-MP001474DZZ
CSB-EP001474DZZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Elongation factor Ts (tsf) CSB-YP025124EYX
CSB-EP025124EYX
CSB-BP025124EYX
CSB-MP025124EYX
CSB-EP025124EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Translation initiation factor IF-2 (infB), partial CSB-YP015164EYX
CSB-EP015164EYX
CSB-BP015164EYX
CSB-MP015164EYX
CSB-EP015164EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Prosthecochloris aestuarii Argininosuccinate synthase (argG) CSB-YP002234EYX
CSB-EP002234EYX
CSB-BP002234EYX
CSB-MP002234EYX
CSB-EP002234EYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Malate dehydrogenase (mdh) CSB-YP013623ETB
CSB-EP013623ETB
CSB-BP013623ETB
CSB-MP013623ETB
CSB-EP013623ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme ATP synthase subunit a (atpB), partial CSB-YP015070ETB1
CSB-EP015070ETB1
CSB-BP015070ETB1
CSB-MP015070ETB1
CSB-EP015070ETB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelodictyon phaeoclathratiforme Lipoyl synthase (lipA) CSB-YP012927ETB
CSB-EP012927ETB
CSB-BP012927ETB
CSB-MP012927ETB
CSB-EP012927ETB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Xaa-Pro dipeptidase (pepQ) CSB-YP017784FLY
CSB-EP017784FLY
CSB-BP017784FLY
CSB-MP017784FLY
CSB-EP017784FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia ATP synthase subunit b (atpF), partial CSB-YP002358FLY1
CSB-EP002358FLY1
CSB-BP002358FLY1
CSB-MP002358FLY1
CSB-EP002358FLY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Peptide deformylase (def) CSB-YP017707FLY
CSB-EP017707FLY
CSB-BP017707FLY
CSB-MP017707FLY
CSB-EP017707FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Peptide chain release factor 1 (prfA) CSB-YP015197FLY
CSB-EP015197FLY
CSB-BP015197FLY
CSB-MP015197FLY
CSB-EP015197FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Phosphoserine aminotransferase (serC) CSB-YP018838FLY
CSB-EP018838FLY
CSB-BP018838FLY
CSB-MP018838FLY
CSB-EP018838FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Triosephosphate isomerase (tpiA) CSB-YP024102FLY
CSB-EP024102FLY
CSB-BP024102FLY
CSB-MP024102FLY
CSB-EP024102FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia Glycine cleavage system H protein (gcvH) CSB-YP009335FLY
CSB-EP009335FLY
CSB-BP009335FLY
CSB-MP009335FLY
CSB-EP009335FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Stenotrophomonas maltophilia GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582FLY
CSB-EP009582FLY
CSB-BP009582FLY
CSB-MP009582FLY
CSB-EP009582FLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Peptide deformylase (def) CSB-YP017707SWS
CSB-EP017707SWS
CSB-BP017707SWS
CSB-MP017707SWS
CSB-EP017707SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SWS
CSB-EP022917SWS
CSB-BP022917SWS
CSB-MP022917SWS
CSB-EP022917SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Triosephosphate isomerase (tpiA) CSB-YP024102SWS
CSB-EP024102SWS
CSB-BP024102SWS
CSB-MP024102SWS
CSB-EP024102SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella newport Malate dehydrogenase (mdh) CSB-YP013623SWS
CSB-EP013623SWS
CSB-BP013623SWS
CSB-MP013623SWS
CSB-EP013623SWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella heidelberg ATP synthase subunit beta (atpD) CSB-YP002350SWQ
CSB-EP002350SWQ
CSB-BP002350SWQ
CSB-MP002350SWQ
CSB-EP002350SWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>