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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methylobacterium nodulans Elongation factor G (fusA), partial CSB-YP009373MTB
CSB-EP009373MTB
CSB-BP009373MTB
CSB-MP009373MTB
CSB-EP009373MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Cardiolipin synthase (cls), partial CSB-YP005985EZY1
CSB-EP005985EZY1
CSB-BP005985EZY1
CSB-MP005985EZY1
CSB-EP005985EZY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa ATP synthase subunit alpha (atpA), partial CSB-YP002344EZY
CSB-EP002344EZY
CSB-BP002344EZY
CSB-MP002344EZY
CSB-EP002344EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582VFH
CSB-EP009582VFH
CSB-BP009582VFH
CSB-MP009582VFH
CSB-EP009582VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Porphobilinogen deaminase (hemC) CSB-YP010524VFH
CSB-EP010524VFH
CSB-BP010524VFH
CSB-MP010524VFH
CSB-EP010524VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Methionyl-tRNA formyltransferase (fmt) CSB-YP015150VFH
CSB-EP015150VFH
CSB-BP015150VFH
CSB-MP015150VFH
CSB-EP015150VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus L-threonine 3-dehydrogenase (tdh) CSB-YP023350VFH
CSB-EP023350VFH
CSB-BP023350VFH
CSB-MP023350VFH
CSB-EP023350VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis LIM domain-containing protein 1 (limd1), partial CSB-YP012949XBF
CSB-EP012949XBF
CSB-BP012949XBF
CSB-MP012949XBF
CSB-EP012949XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Serine hydroxymethyltransferase (glyA) CSB-YP021274SYW
CSB-EP021274SYW
CSB-BP021274SYW
CSB-MP021274SYW
CSB-EP021274SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Translation initiation factor IF-2 (infB), partial CSB-YP015164SYW
CSB-EP015164SYW
CSB-BP015164SYW
CSB-MP015164SYW
CSB-EP015164SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Lipoyl synthase (lipA) CSB-YP012927SYW
CSB-EP012927SYW
CSB-BP012927SYW
CSB-MP012927SYW
CSB-EP012927SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Malate dehydrogenase (mdh) CSB-YP013623SYW
CSB-EP013623SYW
CSB-BP013623SYW
CSB-MP013623SYW
CSB-EP013623SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans ATP synthase subunit alpha (atpA), partial CSB-YP002344SYW
CSB-EP002344SYW
CSB-BP002344SYW
CSB-MP002344SYW
CSB-EP002344SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Ribosome-recycling factor (frr) CSB-YP014941HUE
CSB-EP014941HUE
CSB-BP014941HUE
CSB-MP014941HUE
CSB-EP014941HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfurococcus kamchatkensis Elongation factor 2 (fusA), partial CSB-YP007434DJT
CSB-EP007434DJT
CSB-BP007434DJT
CSB-MP007434DJT
CSB-EP007434DJT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP synthase subunit alpha (atpA), partial CSB-YP002344BXA
CSB-EP002344BXA
CSB-BP002344BXA
CSB-MP002344BXA
CSB-EP002344BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Lipoyl synthase (lipA) CSB-YP012927BXA
CSB-EP012927BXA
CSB-BP012927BXA
CSB-MP012927BXA
CSB-EP012927BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Cysteine desulfurase (iscS) CSB-YP015769BWY
CSB-EP015769BWY
CSB-BP015769BWY
CSB-MP015769BWY
CSB-EP015769BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum ATP synthase subunit alpha (atpA), partial CSB-YP002344BWY
CSB-EP002344BWY
CSB-BP002344BWY
CSB-MP002344BWY
CSB-EP002344BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451BWY
CSB-EP015451BWY
CSB-BP015451BWY
CSB-MP015451BWY
CSB-EP015451BWY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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