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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli O7:K1 Cardiolipin synthase (cls), partial CSB-YP005985EON1
CSB-EP005985EON1
CSB-BP005985EON1
CSB-MP005985EON1
CSB-EP005985EON1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 GMP synthase [glutamine-hydrolyzing] (guaA) CSB-YP009582EOB
CSB-EP009582EOB
CSB-BP009582EOB
CSB-MP009582EOB
CSB-EP009582EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Nucleoside diphosphate kinase (ndk) CSB-YP015889EOB
CSB-EP015889EOB
CSB-BP015889EOB
CSB-MP015889EOB
CSB-EP015889EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Ribonuclease 3 (rnc) CSB-YP019809EOB
CSB-EP019809EOB
CSB-BP019809EOB
CSB-MP019809EOB
CSB-EP019809EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Thymidylate kinase (tmk) CSB-YP007226EOB
CSB-EP007226EOB
CSB-BP007226EOB
CSB-MP007226EOB
CSB-EP007226EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O127:H6 Translation initiation factor IF-3 (infC) CSB-YP015166EOB
CSB-EP015166EOB
CSB-BP015166EOB
CSB-MP015166EOB
CSB-EP015166EOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA dimethylallyltransferase (miaA) CSB-YP024529EZY
CSB-EP024529EZY
CSB-BP024529EZY
CSB-MP024529EZY
CSB-EP024529EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus tRNA dimethylallyltransferase (miaA) CSB-YP024529VFH
CSB-EP024529VFH
CSB-BP024529VFH
CSB-MP024529VFH
CSB-EP024529VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Elongation factor Ts (tsf) CSB-YP025124VFH
CSB-EP025124VFH
CSB-BP025124VFH
CSB-MP025124VFH
CSB-EP025124VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Thymidylate synthase (thyA) CSB-YP025393VFH
CSB-EP025393VFH
CSB-BP025393VFH
CSB-MP025393VFH
CSB-EP025393VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Ribonuclease 3 (rnc) CSB-YP019809VFH
CSB-EP019809VFH
CSB-BP019809VFH
CSB-MP019809VFH
CSB-EP019809VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Protein N-terminal glutamine amidohydrolase (OsI_19806) CSB-YP026083OFF
CSB-EP026083OFF
CSB-BP026083OFF
CSB-MP026083OFF
CSB-EP026083OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Nucleoside diphosphate kinase (ndk) CSB-YP015889SYW
CSB-EP015889SYW
CSB-BP015889SYW
CSB-MP015889SYW
CSB-EP015889SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Thymidylate synthase (thyA) CSB-YP025393SYW
CSB-EP025393SYW
CSB-BP025393SYW
CSB-MP025393SYW
CSB-EP025393SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Transaldolase (tal) CSB-YP023112SYW
CSB-EP023112SYW
CSB-BP023112SYW
CSB-MP023112SYW
CSB-EP023112SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans ATP synthase subunit a (atpB), partial CSB-YP015070SYW1
CSB-EP015070SYW1
CSB-BP015070SYW1
CSB-MP015070SYW1
CSB-EP015070SYW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Elongation factor Ts (tsf) CSB-YP025124HUE
CSB-EP025124HUE
CSB-BP025124HUE
CSB-MP025124HUE
CSB-EP025124HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii Methionyl-tRNA formyltransferase (fmt) CSB-YP015150HUE
CSB-EP015150HUE
CSB-BP015150HUE
CSB-MP015150HUE
CSB-EP015150HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582HUE
CSB-EP009582HUE
CSB-BP009582HUE
CSB-MP009582HUE
CSB-EP009582HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfurococcus kamchatkensis Protein pelota homolog (pelA) CSB-YP017778DJT
CSB-EP017778DJT
CSB-BP017778DJT
CSB-MP017778DJT
CSB-EP017778DJT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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