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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acidithiobacillus ferrooxidans Elongation factor G (fusA), partial CSB-YP009373AWI
CSB-EP009373AWI
CSB-BP009373AWI
CSB-MP009373AWI
CSB-EP009373AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SWK
CSB-EP019146SWK
CSB-BP019146SWK
CSB-MP019146SWK
CSB-EP019146SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona ATP synthase subunit a (atpB), partial CSB-YP015070SWK1
CSB-EP015070SWK1
CSB-BP015070SWK1
CSB-MP015070SWK1
CSB-EP015070SWK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 3-ketoacyl-CoA thiolase (fadI) CSB-YP001117SWK
CSB-EP001117SWK
CSB-BP001117SWK
CSB-MP001117SWK
CSB-EP001117SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Galactokinase (galK) CSB-YP009198SWK
CSB-EP009198SWK
CSB-BP009198SWK
CSB-MP009198SWK
CSB-EP009198SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona 6-phosphofructokinase (pfkA) CSB-YP017822SWK
CSB-EP017822SWK
CSB-BP017822SWK
CSB-MP017822SWK
CSB-EP017822SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146VFC
CSB-EP019146VFC
CSB-BP019146VFC
CSB-MP019146VFC
CSB-EP019146VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Argininosuccinate synthase (argG) CSB-YP002234VFC
CSB-EP002234VFC
CSB-BP002234VFC
CSB-MP002234VFC
CSB-EP002234VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Ferrochelatase (hemH) CSB-YP008579VFC
CSB-EP008579VFC
CSB-BP008579VFC
CSB-MP008579VFC
CSB-EP008579VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphoserine aminotransferase (serC) CSB-YP018838VFC
CSB-EP018838VFC
CSB-BP018838VFC
CSB-MP018838VFC
CSB-EP018838VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Peptide deformylase (def) CSB-YP017707VFC
CSB-EP017707VFC
CSB-BP017707VFC
CSB-MP017707VFC
CSB-EP017707VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri ATP synthase subunit a (atpB), partial CSB-YP015070VFC1
CSB-EP015070VFC1
CSB-BP015070VFC1
CSB-MP015070VFC1
CSB-EP015070VFC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri NAD-dependent malic enzyme (maeA), partial CSB-YP013633VFC
CSB-EP013633VFC
CSB-BP013633VFC
CSB-MP013633VFC
CSB-EP013633VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451SWN
CSB-EP015451SWN
CSB-BP015451SWN
CSB-MP015451SWN
CSB-EP015451SWN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella dublin Cardiolipin synthase (cls), partial CSB-YP005985SWN1
CSB-EP005985SWN1
CSB-BP005985SWN1
CSB-MP005985SWN1
CSB-EP005985SWN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Otolemur garnettii Histone-lysine N-methyltransferase setd3 (SETD3), partial CSB-YP021104OFS
CSB-EP021104OFS
CSB-BP021104OFS
CSB-MP021104OFS
CSB-EP021104OFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taeniopygia guttata ADP-ribosylation factor-like protein 3 (ARL3) CSB-YP002084TKW
CSB-EP002084TKW
CSB-BP002084TKW
CSB-MP002084TKW
CSB-EP002084TKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taeniopygia guttata Eukaryotic initiation factor 4A-III (EIF4A3) CSB-YP007553TKW
CSB-EP007553TKW
CSB-BP007553TKW
CSB-MP007553TKW
CSB-EP007553TKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SWO
CSB-EP019146SWO
CSB-BP019146SWO
CSB-MP019146SWO
CSB-EP019146SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella enteritidis PT4 NAD-dependent malic enzyme (maeA), partial CSB-YP013633SWO
CSB-EP013633SWO
CSB-BP013633SWO
CSB-MP013633SWO
CSB-EP013633SWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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