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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhizobium leguminosarum bv. trifolii tRNA pseudouridine synthase A (truA) CSB-YP019086RKQ
CSB-EP019086RKQ
CSB-BP019086RKQ
CSB-MP019086RKQ
CSB-EP019086RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717RKQ
CSB-EP009717RKQ
CSB-BP009717RKQ
CSB-MP009717RKQ
CSB-EP009717RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii ATP synthase subunit a (atpB), partial CSB-YP015070RKQ1
CSB-EP015070RKQ1
CSB-BP015070RKQ1
CSB-MP015070RKQ1
CSB-EP015070RKQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Malate dehydrogenase (mdh) CSB-YP013623RKQ
CSB-EP013623RKQ
CSB-BP013623RKQ
CSB-MP013623RKQ
CSB-EP013623RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033RKQ
CSB-EP018033RKQ
CSB-BP018033RKQ
CSB-MP018033RKQ
CSB-EP018033RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Nucleoside diphosphate kinase (ndk) CSB-YP015889RKQ
CSB-EP015889RKQ
CSB-BP015889RKQ
CSB-MP015889RKQ
CSB-EP015889RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium leguminosarum bv. trifolii Translation initiation factor IF-3 (infC) CSB-YP015166RKQ
CSB-EP015166RKQ
CSB-BP015166RKQ
CSB-MP015166RKQ
CSB-EP015166RKQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917AZM
CSB-EP022917AZM
CSB-BP022917AZM
CSB-MP022917AZM
CSB-EP022917AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Peptide deformylase (def) CSB-YP017707AZM
CSB-EP017707AZM
CSB-BP017707AZM
CSB-MP017707AZM
CSB-EP017707AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida Porphobilinogen deaminase (hemC) CSB-YP010524AZM
CSB-EP010524AZM
CSB-BP010524AZM
CSB-MP010524AZM
CSB-EP010524AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aliivibrio salmonicida L-threonine 3-dehydrogenase (tdh) CSB-YP023350AZM
CSB-EP023350AZM
CSB-BP023350AZM
CSB-MP023350AZM
CSB-EP023350AZM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Argininosuccinate synthase (argG) CSB-YP002234ENW
CSB-EP002234ENW
CSB-BP002234ENW
CSB-MP002234ENW
CSB-EP002234ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetylneuraminate lyase (nanA) CSB-YP015994ENW
CSB-EP015994ENW
CSB-BP015994ENW
CSB-MP015994ENW
CSB-EP015994ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide deformylase (def) CSB-YP017707ENW
CSB-EP017707ENW
CSB-BP017707ENW
CSB-MP017707ENW
CSB-EP017707ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Elongation factor G (fusA) CSB-YP009373ENW
CSB-EP009373ENW
CSB-BP009373ENW
CSB-MP009373ENW
CSB-EP009373ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase subunit delta (atpH) CSB-YP002355ENW
CSB-EP002355ENW
CSB-BP002355ENW
CSB-MP002355ENW
CSB-EP002355ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glucose-6-phosphate isomerase (pgi) CSB-YP009717ENW
CSB-EP009717ENW
CSB-BP009717ENW
CSB-MP009717ENW
CSB-EP009717ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Galactokinase (galK) CSB-YP009198ENW
CSB-EP009198ENW
CSB-BP009198ENW
CSB-MP009198ENW
CSB-EP009198ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451ENW
CSB-EP015451ENW
CSB-BP015451ENW
CSB-MP015451ENW
CSB-EP015451ENW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodospirillum centenum Serine hydroxymethyltransferase (glyA) CSB-YP021274RLY
CSB-EP021274RLY
CSB-BP021274RLY
CSB-MP021274RLY
CSB-EP021274RLY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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