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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis Aspartate beta-hydroxylase domain-containing protein 2 (asphd2), partial CSB-YP002228XBF1
CSB-EP002228XBF1
CSB-BP002228XBF1
CSB-MP002228XBF1
CSB-EP002228XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Mitochondrial ribonuclease P protein 3 (Mrpp3) CSB-YP012187RA
CSB-EP012187RA
CSB-BP012187RA
CSB-MP012187RA
CSB-EP012187RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo salar RNA-binding protein 8A (rbm8a) CSB-YP019437SWI
CSB-EP019437SWI
CSB-BP019437SWI
CSB-MP019437SWI
CSB-EP019437SWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Protein arginine N-methyltransferase 7 (Art7), partial CSB-YP018736DME
CSB-EP018736DME
CSB-BP018736DME
CSB-MP018736DME
CSB-EP018736DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila pseudoobscura pseudoobscura Protein SMG8 (GA24994), partial CSB-YP003375DME
CSB-EP003375DME
CSB-BP003375DME
CSB-MP003375DME
CSB-EP003375DME-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Acyl carrier protein (acpP) CSB-YP015636GFN
CSB-EP015636GFN
CSB-BP015636GFN
CSB-MP015636GFN
CSB-EP015636GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter bemidjiensis Ribosome-recycling factor (frr) CSB-YP014941GFN
CSB-EP014941GFN
CSB-BP014941GFN
CSB-MP014941GFN
CSB-EP014941GFN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Serine hydroxymethyltransferase (glyA) CSB-YP021274AWI
CSB-EP021274AWI
CSB-BP021274AWI
CSB-MP021274AWI
CSB-EP021274AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidithiobacillus ferrooxidans Porphobilinogen deaminase (hemC) CSB-YP010524AWI
CSB-EP010524AWI
CSB-BP010524AWI
CSB-MP010524AWI
CSB-EP010524AWI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Galactokinase (galK) CSB-YP009198VFC
CSB-EP009198VFC
CSB-BP009198VFC
CSB-MP009198VFC
CSB-EP009198VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona L-threonine 3-dehydrogenase (tdh) CSB-YP023350SWK
CSB-EP023350SWK
CSB-BP023350SWK
CSB-MP023350SWK
CSB-EP023350SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Glycine dehydrogenase [decarboxylating] (gcvP), partial CSB-YP009493SWK
CSB-EP009493SWK
CSB-BP009493SWK
CSB-MP009493SWK
CSB-EP009493SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Argininosuccinate synthase (argG) CSB-YP002234SWK
CSB-EP002234SWK
CSB-BP002234SWK
CSB-MP002234SWK
CSB-EP002234SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Malate dehydrogenase (mdh) CSB-YP013623SWK
CSB-EP013623SWK
CSB-BP013623SWK
CSB-MP013623SWK
CSB-EP013623SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Methionyl-tRNA formyltransferase (fmt) CSB-YP015150SWK
CSB-EP015150SWK
CSB-BP015150SWK
CSB-MP015150SWK
CSB-EP015150SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella agona Thymidylate kinase (tmk) CSB-YP007226SWK
CSB-EP007226SWK
CSB-BP007226SWK
CSB-MP007226SWK
CSB-EP007226SWK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Elongation factor Ts (tsf) CSB-YP025124VFC
CSB-EP025124VFC
CSB-BP025124VFC
CSB-MP025124VFC
CSB-EP025124VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582VFC
CSB-EP009582VFC
CSB-BP009582VFC
CSB-MP009582VFC
CSB-EP009582VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri Phosphatidylserine decarboxylase proenzyme (psd) CSB-YP018033VFC
CSB-EP018033VFC
CSB-BP018033VFC
CSB-MP018033VFC
CSB-EP018033VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio fischeri 6-phosphofructokinase (pfkA) CSB-YP017822VFC
CSB-EP017822VFC
CSB-BP017822VFC
CSB-MP017822VFC
CSB-EP017822VFC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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