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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanosphaerula palustris FAD synthase (ribL) CSB-YP008710MSP
CSB-EP008710MSP
CSB-BP008710MSP
CSB-MP008710MSP
CSB-EP008710MSP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. Lipoyl synthase (lipA) CSB-YP012927TNX
CSB-EP012927TNX
CSB-BP012927TNX
CSB-MP012927TNX
CSB-EP012927TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thioalkalivibrio sp. Porphobilinogen deaminase (hemC) CSB-YP010524TNX
CSB-EP010524TNX
CSB-BP010524TNX
CSB-MP010524TNX
CSB-EP010524TNX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DQP
CSB-EP009582DQP
CSB-BP009582DQP
CSB-MP009582DQP
CSB-EP009582DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Ferrochelatase (hemH) CSB-YP008579DQP
CSB-EP008579DQP
CSB-BP008579DQP
CSB-MP008579DQP
CSB-EP008579DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Glucokinase (glk) CSB-YP009319DQP
CSB-EP009319DQP
CSB-BP009319DQP
CSB-MP009319DQP
CSB-EP009319DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717DQP
CSB-EP009717DQP
CSB-BP009717DQP
CSB-MP009717DQP
CSB-EP009717DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus ATP synthase subunit alpha (atpA), partial CSB-YP002344DQP
CSB-EP002344DQP
CSB-BP002344DQP
CSB-MP002344DQP
CSB-EP002344DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Ribosome-recycling factor (frr) CSB-YP014941DOG
CSB-EP014941DOG
CSB-BP014941DOG
CSB-MP014941DOG
CSB-EP014941DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Ferrochelatase (hemH) CSB-YP008579EPV
CSB-EP008579EPV
CSB-BP008579EPV
CSB-MP008579EPV
CSB-EP008579EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Elongation factor Ts (tsf) CSB-YP025124EPV
CSB-EP025124EPV
CSB-BP025124EPV
CSB-MP025124EPV
CSB-EP025124EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Peptide chain release factor 1 (prfA) CSB-YP015197EPV
CSB-EP015197EPV
CSB-BP015197EPV
CSB-MP015197EPV
CSB-EP015197EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582DUL
CSB-EP009582DUL
CSB-BP009582DUL
CSB-MP009582DUL
CSB-EP009582DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Porphobilinogen deaminase (hemC) CSB-YP010524EZY
CSB-EP010524EZY
CSB-BP010524EZY
CSB-MP010524EZY
CSB-EP010524EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ribosome-recycling factor (frr) CSB-YP014941EZY
CSB-EP014941EZY
CSB-BP014941EZY
CSB-MP014941EZY
CSB-EP014941EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Argininosuccinate synthase (argG) CSB-YP002234SYW
CSB-EP002234SYW
CSB-BP002234SYW
CSB-MP002234SYW
CSB-EP002234SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii GTPase Era (era) CSB-YP007759HUE
CSB-EP007759HUE
CSB-BP007759HUE
CSB-MP007759HUE
CSB-EP007759HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halothermothrix orenii ATP synthase subunit delta (atpH) CSB-YP002355HUE
CSB-EP002355HUE
CSB-BP002355HUE
CSB-MP002355HUE
CSB-EP002355HUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Transaldolase (tal) CSB-YP023112BXA
CSB-EP023112BXA
CSB-BP023112BXA
CSB-MP023112BXA
CSB-EP023112BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Translation initiation factor IF-3 (infC) CSB-YP015166BXA
CSB-EP015166BXA
CSB-BP015166BXA
CSB-MP015166BXA
CSB-EP015166BXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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