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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Caulobacter crescentus Urocanate hydratase (hutU), partial CSB-YP025677DQP
CSB-EP025677DQP
CSB-BP025677DQP
CSB-MP025677DQP
CSB-EP025677DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Translation initiation factor IF-3 (infC) CSB-YP015166DQP
CSB-EP015166DQP
CSB-BP015166DQP
CSB-MP015166DQP
CSB-EP015166DQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824DOG
CSB-EP005824DOG
CSB-BP005824DOG
CSB-MP005824DOG
CSB-EP005824DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter chlorophenolicus Elongation factor Tu (tuf) CSB-YP025342DOG
CSB-EP025342DOG
CSB-BP025342DOG
CSB-MP025342DOG
CSB-EP025342DOG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyanothece sp. Methionyl-tRNA formyltransferase (fmt) CSB-YP015150EPV
CSB-EP015150EPV
CSB-BP015150EPV
CSB-MP015150EPV
CSB-EP015150EPV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium cellulolyticum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150DUL
CSB-EP015150DUL
CSB-BP015150DUL
CSB-MP015150DUL
CSB-EP015150DUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Thymidylate synthase (thyA) CSB-YP025393MTB
CSB-EP025393MTB
CSB-BP025393MTB
CSB-MP025393MTB
CSB-EP025393MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans Nucleoside diphosphate kinase (ndk) CSB-YP015889MTB
CSB-EP015889MTB
CSB-BP015889MTB
CSB-MP015889MTB
CSB-EP015889MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium nodulans ATP synthase subunit alpha (atpA), partial CSB-YP002344MTB
CSB-EP002344MTB
CSB-BP002344MTB
CSB-MP002344MTB
CSB-EP002344MTB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Triosephosphate isomerase (tpiA) CSB-YP024102EZY
CSB-EP024102EZY
CSB-BP024102EZY
CSB-MP024102EZY
CSB-EP024102EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Phosphoserine aminotransferase (serC) CSB-YP018838EZY
CSB-EP018838EZY
CSB-BP018838EZY
CSB-MP018838EZY
CSB-EP018838EZY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717VFH
CSB-EP009717VFH
CSB-BP009717VFH
CSB-MP009717VFH
CSB-EP009717VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146VFH
CSB-EP019146VFH
CSB-BP019146VFH
CSB-MP019146VFH
CSB-EP019146VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Peptide chain release factor 1 (prfA) CSB-YP015197VFH
CSB-EP015197VFH
CSB-BP015197VFH
CSB-MP015197VFH
CSB-EP015197VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio splendidus Urocanate hydratase (hutU), partial CSB-YP025677VFH
CSB-EP025677VFH
CSB-BP025677VFH
CSB-MP025677VFH
CSB-EP025677VFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Swi5-dependent recombination DNA repair protein 1 homolog (sfr1) CSB-YP002975DIL
CSB-EP002975DIL
CSB-BP002975DIL
CSB-MP002975DIL
CSB-EP002975DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis General transcription factor II-I repeat domain-containing protein 1 (gtf2ird1), partial CSB-YP010018XBE
CSB-EP010018XBE
CSB-BP010018XBE
CSB-MP010018XBE
CSB-EP010018XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Eukaryotic initiation factor 4A-III (eif4a3) CSB-YP007553XBF
CSB-EP007553XBF
CSB-BP007553XBF
CSB-MP007553XBF
CSB-EP007553XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Putative ATP-dependent RNA helicase TDRD9 (tdrd9), partial CSB-YP023361DIL
CSB-EP023361DIL
CSB-BP023361DIL
CSB-MP023361DIL
CSB-EP023361DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella piezotolerans Triosephosphate isomerase (tpiA) CSB-YP024102SYW
CSB-EP024102SYW
CSB-BP024102SYW
CSB-MP024102SYW
CSB-EP024102SYW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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