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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Saccharomyces cerevisiae Enolase-phosphatase E1 (UTR4) CSB-YP007674SVL
CSB-EP007674SVL
CSB-BP007674SVL
CSB-MP007674SVL
CSB-EP007674SVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Flap endonuclease 1 (RAD27) CSB-YP008585SVL
CSB-EP008585SVL
CSB-BP008585SVL
CSB-MP008585SVL
CSB-EP008585SVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acidimicrobium ferrooxidans GTPase Era (era) CSB-YP007759AWC
CSB-EP007759AWC
CSB-BP007759AWC
CSB-MP007759AWC
CSB-EP007759AWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nectria haematococca U1 small nuclear ribonucleoprotein C (NECHADRAFT_80502) CSB-YP022331NFS
CSB-EP022331NFS
CSB-BP022331NFS
CSB-MP022331NFS
CSB-EP022331NFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Eukaryotic translation initiation factor 3 subunit G (tif35) CSB-YP007536EKF
CSB-EP007536EKF
CSB-BP007536EKF
CSB-MP007536EKF
CSB-EP007536EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Inosine triphosphate pyrophosphatase (AN8861) CSB-YP011907EKF
CSB-EP011907EKF
CSB-BP011907EKF
CSB-MP011907EKF
CSB-EP011907EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Aquaporin-1 (AQY1), partial CSB-YP001957SVN1
CSB-EP001957SVN1
CSB-BP001957SVN1
CSB-MP001957SVN1
CSB-EP001957SVN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana CST complex subunit CTC1 (CTC1), partial CSB-YP003371DOA
CSB-EP003371DOA
CSB-BP003371DOA
CSB-MP003371DOA
CSB-EP003371DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phytophthora infestans Alpha-tubulin N-acetyltransferase (PITG_13069) CSB-YP004059EVF
CSB-EP004059EVF
CSB-BP004059EVF
CSB-MP004059EVF
CSB-EP004059EVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca DnaJ homolog subfamily C member 5B (DNAJC5B) CSB-YP007038AYX
CSB-EP007038AYX
CSB-BP007038AYX
CSB-MP007038AYX
CSB-EP007038AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca E3 ubiquitin-protein ligase RNF182 (RNF182), partial CSB-YP019861AYX1
CSB-EP019861AYX1
CSB-BP019861AYX1
CSB-MP019861AYX1
CSB-EP019861AYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Leucine-rich repeat and fibronectin type-III domain-containing protein 3 (LRFN3), partial CSB-YP013079AYX1
CSB-EP013079AYX1
CSB-BP013079AYX1
CSB-MP013079AYX1
CSB-EP013079AYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Actin-related protein 8 (ACTR8), partial CSB-YP001254AYX
CSB-EP001254AYX
CSB-BP001254AYX
CSB-MP001254AYX
CSB-EP001254AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium eligens Methionyl-tRNA formyltransferase (fmt) CSB-YP015150EOW
CSB-EP015150EOW
CSB-BP015150EOW
CSB-MP015150EOW
CSB-EP015150EOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium rectale Argininosuccinate synthase (argG) CSB-YP002234EOX
CSB-EP002234EOX
CSB-BP002234EOX
CSB-MP002234EOX
CSB-EP002234EOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium rectale Peptide deformylase (def) CSB-YP017707EOX
CSB-EP017707EOX
CSB-BP017707EOX
CSB-MP017707EOX
CSB-EP017707EOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium rectale Serine hydroxymethyltransferase (glyA) CSB-YP021274EOX
CSB-EP021274EOX
CSB-BP021274EOX
CSB-MP021274EOX
CSB-EP021274EOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA dimethylallyltransferase (miaA) CSB-YP024529ENU
CSB-EP024529ENU
CSB-BP024529ENU
CSB-MP024529ENU
CSB-EP024529ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-ketoacyl-CoA thiolase (fadI) CSB-YP001117ENU
CSB-EP001117ENU
CSB-BP001117ENU
CSB-MP001117ENU
CSB-EP001117ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Lipoyl synthase (lipA) CSB-YP012927ENU
CSB-EP012927ENU
CSB-BP012927ENU
CSB-MP012927ENU
CSB-EP012927ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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