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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Sorghum bicolor Inosine triphosphate pyrophosphatase (Sb01g020160) CSB-YP011907FJS
CSB-EP011907FJS
CSB-BP011907FJS
CSB-MP011907FJS
CSB-EP011907FJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus sibiricus Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614TMT
CSB-EP017614TMT
CSB-BP017614TMT
CSB-MP017614TMT
CSB-EP017614TMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio salexigens Porphobilinogen deaminase (hemC) CSB-YP010524DJQ
CSB-EP010524DJQ
CSB-BP010524DJQ
CSB-MP010524DJQ
CSB-EP010524DJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio salexigens Peptide chain release factor 1 (prfA) CSB-YP015197DJQ
CSB-EP015197DJQ
CSB-BP015197DJQ
CSB-MP015197DJQ
CSB-EP015197DJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfovibrio salexigens Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799DJQ
CSB-EP014799DJQ
CSB-BP014799DJQ
CSB-MP014799DJQ
CSB-EP014799DJQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Ribosome-recycling factor (frr) CSB-YP014941ESQ
CSB-EP014941ESQ
CSB-BP014941ESQ
CSB-MP014941ESQ
CSB-EP014941ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Ribonuclease 3 (rnc) CSB-YP019809ESQ
CSB-EP019809ESQ
CSB-BP019809ESQ
CSB-MP019809ESQ
CSB-EP019809ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917ESQ
CSB-EP022917ESQ
CSB-BP022917ESQ
CSB-MP022917ESQ
CSB-EP022917ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Phosphoserine aminotransferase (serC) CSB-YP018838ESQ
CSB-EP018838ESQ
CSB-BP018838ESQ
CSB-MP018838ESQ
CSB-EP018838ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150ESQ
CSB-EP015150ESQ
CSB-BP015150ESQ
CSB-MP015150ESQ
CSB-EP015150ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum ATP synthase subunit a (atpB), partial CSB-YP015070ESQ1
CSB-EP015070ESQ1
CSB-BP015070ESQ1
CSB-MP015070ESQ1
CSB-EP015070ESQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pectobacterium carotovorum subsp. carotovorum Translation initiation factor IF-2 (infB), partial CSB-YP015164ESQ
CSB-EP015164ESQ
CSB-BP015164ESQ
CSB-MP015164ESQ
CSB-EP015164ESQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sp. Thymidylate kinase (tmk) CSB-YP007226GFR
CSB-EP007226GFR
CSB-BP007226GFR
CSB-MP007226GFR
CSB-EP007226GFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sp. Ferrochelatase (hemH) CSB-YP008579GFR
CSB-EP008579GFR
CSB-BP008579GFR
CSB-MP008579GFR
CSB-EP008579GFR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Methionine aminopeptidase 2 (MAP2) CSB-YP013716SVL
CSB-EP013716SVL
CSB-BP013716SVL
CSB-MP013716SVL
CSB-EP013716SVL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhabdus utahensis FAD synthase (ribL) CSB-YP008710HUA
CSB-EP008710HUA
CSB-BP008710HUA
CSB-MP008710HUA
CSB-EP008710HUA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nectria haematococca Inosine triphosphate pyrophosphatase (NECHADRAFT_105230) CSB-YP011907NFS
CSB-EP011907NFS
CSB-BP011907NFS
CSB-MP011907NFS
CSB-EP011907NFS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pteropus giganteus Hemoglobin subunit alpha (HBA) CSB-YP010147FHG
CSB-EP010147FHG
CSB-BP010147FHG
CSB-MP010147FHG
CSB-EP010147FHG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sordaria macrospora Flap endonuclease 1 (FEN1) CSB-YP008585FIM
CSB-EP008585FIM
CSB-BP008585FIM
CSB-MP008585FIM
CSB-EP008585FIM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Double-stranded RNA-binding protein Staufen homolog 1 (STAU1), partial CSB-YP022818AYX
CSB-EP022818AYX
CSB-BP022818AYX
CSB-MP022818AYX
CSB-EP022818AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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