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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Phosphoglucomutase-2 (pp63-2), partial CSB-YP017867ERW
CSB-EP017867ERW
CSB-BP017867ERW
CSB-MP017867ERW
CSB-EP017867ERW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca nemestrina Receptor-type tyrosine-protein phosphatase N2 (PTPRN2), partial CSB-YP019060MOX
CSB-EP019060MOX
CSB-BP019060MOX
CSB-MP019060MOX
CSB-EP019060MOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Monodelphis domestica DNA nucleotidylexotransferase (DNTT) CSB-YP007088MUE
CSB-EP007088MUE
CSB-BP007088MUE
CSB-MP007088MUE
CSB-EP007088MUE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae NADH-ubiquinone oxidoreductase chain 2 (MT-ND2), partial CSB-YP015077LNS
CSB-EP015077LNS
CSB-BP015077LNS
CSB-MP015077LNS
CSB-EP015077LNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae Cytochrome c oxidase subunit 3 (MT-CO3), partial CSB-YP015074LNS1
CSB-EP015074LNS1
CSB-BP015074LNS1
CSB-MP015074LNS1
CSB-EP015074LNS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072DRG
CSB-EP015072DRG
CSB-BP015072DRG
CSB-MP015072DRG
CSB-EP015072DRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ceratotherium simum NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DRG
CSB-EP015079DRG
CSB-BP015079DRG
CSB-MP015079DRG
CSB-EP015079DRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo trutta NADH-ubiquinone oxidoreductase chain 3 (MT-ND3), partial CSB-YP015078SWJ1
CSB-EP015078SWJ1
CSB-BP015078SWJ1
CSB-MP015078SWJ1
CSB-EP015078SWJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Cytochrome b (MT-CYB), partial CSB-YP015075TQG
CSB-EP015075TQG
CSB-BP015075TQG
CSB-MP015075TQG
CSB-EP015075TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nothoprocta perdicaria Cytochrome b (MT-CYB), partial CSB-YP015075NHU
CSB-EP015075NHU
CSB-BP015075NHU
CSB-MP015075NHU
CSB-EP015075NHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhea americana Cytochrome c oxidase subunit 1 (MT-CO1), partial CSB-YP015072RKB
CSB-EP015072RKB
CSB-BP015072RKB
CSB-MP015072RKB
CSB-EP015072RKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bothriopsis bilineata NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079BUQ1
CSB-EP015079BUQ1
CSB-BP015079BUQ1
CSB-MP015079BUQ1
CSB-EP015079BUQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Causus rhombeatus NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079DQS1
CSB-EP015079DQS1
CSB-BP015079DQS1
CSB-MP015079DQS1
CSB-EP015079DQS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hypnale hypnale NADH-ubiquinone oxidoreductase chain 4 (MT-ND4), partial CSB-YP015079HYD1
CSB-EP015079HYD1
CSB-BP015079HYD1
CSB-MP015079HYD1
CSB-EP015079HYD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kogia breviceps Cytochrome b (MT-CYB), partial CSB-YP015075KBP
CSB-EP015075KBP
CSB-BP015075KBP
CSB-MP015075KBP
CSB-EP015075KBP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Latimeria chalumnae Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073LNS1
CSB-EP015073LNS1
CSB-BP015073LNS1
CSB-MP015073LNS1
CSB-EP015073LNS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nothoprocta perdicaria Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073NHU1
CSB-EP015073NHU1
CSB-BP015073NHU1
CSB-MP015073NHU1
CSB-EP015073NHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana tabacum S-adenosylmethionine decarboxylase proenzyme (SAMDC) CSB-YP001658NHE
CSB-EP001658NHE
CSB-BP001658NHE
CSB-MP001658NHE
CSB-EP001658NHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Importin subunit alpha-2 (KAP2), partial CSB-YP012484DOA
CSB-EP012484DOA
CSB-BP012484DOA
CSB-MP012484DOA
CSB-EP012484DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Tubulin-specific chaperone A (At2g30410) CSB-YP023225DOA
CSB-EP023225DOA
CSB-BP023225DOA
CSB-MP023225DOA
CSB-EP023225DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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