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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Ailuropoda melanoleuca Alpha N-terminal protein methyltransferase 1A (METTL11A) CSB-YP013725AYX
CSB-EP013725AYX
CSB-BP013725AYX
CSB-MP013725AYX
CSB-EP013725AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca E3 ubiquitin-protein ligase RNF152 (RNF152), partial CSB-YP019848AYX1
CSB-EP019848AYX1
CSB-BP019848AYX1
CSB-MP019848AYX1
CSB-EP019848AYX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Kelch-like protein 22 (KLHL22), partial CSB-YP012424AYX
CSB-EP012424AYX
CSB-BP012424AYX
CSB-MP012424AYX
CSB-EP012424AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Zinc finger and SCAN domain-containing protein 4 (ZSCAN4) CSB-YP027141AYX
CSB-EP027141AYX
CSB-BP027141AYX
CSB-MP027141AYX
CSB-EP027141AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ailuropoda melanoleuca Serine/threonine-protein kinase greatwall (MASTL), partial CSB-YP013515AYX
CSB-EP013515AYX
CSB-BP013515AYX
CSB-MP013515AYX
CSB-EP013515AYX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haloterrigena turkmenica FAD synthase (ribL) CSB-YP008710HUD
CSB-EP008710HUD
CSB-BP008710HUD
CSB-MP008710HUD
CSB-EP008710HUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Naegleria gruberi Probable methylthioribulose-1-phosphate dehydratase (NAEGRDRAFT_70372) CSB-YP001906NFF
CSB-EP001906NFF
CSB-BP001906NFF
CSB-MP001906NFF
CSB-EP001906NFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eubacterium rectale Methionyl-tRNA formyltransferase (fmt) CSB-YP015150EOX
CSB-EP015150EOX
CSB-BP015150EOX
CSB-MP015150EOX
CSB-EP015150EOX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-acetylneuraminate lyase (nanA) CSB-YP015994ENU
CSB-EP015994ENU
CSB-BP015994ENU
CSB-MP015994ENU
CSB-EP015994ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide chain release factor 1 (prfA) CSB-YP015197ENU
CSB-EP015197ENU
CSB-BP015197ENU
CSB-MP015197ENU
CSB-EP015197ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide deformylase (def) CSB-YP017707ENU
CSB-EP017707ENU
CSB-BP017707ENU
CSB-MP017707ENU
CSB-EP017707ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Serine hydroxymethyltransferase (glyA) CSB-YP021274ENU
CSB-EP021274ENU
CSB-BP021274ENU
CSB-MP021274ENU
CSB-EP021274ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase subunit beta (atpD) CSB-YP002350ENU
CSB-EP002350ENU
CSB-BP002350ENU
CSB-MP002350ENU
CSB-EP002350ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Thymidylate synthase (thyA) CSB-YP025393ENU
CSB-EP025393ENU
CSB-BP025393ENU
CSB-MP025393ENU
CSB-EP025393ENU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658TMP
CSB-EP001658TMP
CSB-BP001658TMP
CSB-MP001658TMP
CSB-EP001658TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans Protein pelota homolog (pelA) CSB-YP017778TMP
CSB-EP017778TMP
CSB-BP017778TMP
CSB-MP017778TMP
CSB-EP017778TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermococcus gammatolerans Elongation factor 2 (fusA), partial CSB-YP007434TMP
CSB-EP007434TMP
CSB-BP007434TMP
CSB-MP007434TMP
CSB-EP007434TMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Glucokinase (glk) CSB-YP009319EJO
CSB-EP009319EJO
CSB-BP009319EJO
CSB-MP009319EJO
CSB-EP009319EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Edwardsiella ictaluri Nicotinate phosphoribosyltransferase (pncB) CSB-YP015451EJO
CSB-EP015451EJO
CSB-BP015451EJO
CSB-MP015451EJO
CSB-EP015451EJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Teredinibacter turnerae Transaldolase (tal) CSB-YP023112TLR
CSB-EP023112TLR
CSB-BP023112TLR
CSB-MP023112TLR
CSB-EP023112TLR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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