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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ictalurus punctatus Cellular tumor antigen p53 (tp53) CSB-YP024077ILX
CSB-EP024077ILX
CSB-BP024077ILX
CSB-MP024077ILX
CSB-EP024077ILX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coryphopterus nicholsi L-lactate dehydrogenase A chain (ldha) CSB-YP012832DXE
CSB-EP012832DXE
CSB-BP012832DXE
CSB-MP012832DXE
CSB-EP012832DXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Harpagifer antarcticus L-lactate dehydrogenase A chain (ldha) CSB-YP012832HUN
CSB-EP012832HUN
CSB-BP012832HUN
CSB-MP012832HUN
CSB-EP012832HUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Champsocephalus gunnari L-lactate dehydrogenase A chain (ldha) CSB-YP012832DRP
CSB-EP012832DRP
CSB-BP012832DRP
CSB-MP012832DRP
CSB-EP012832DRP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepidonotothen nudifrons L-lactate dehydrogenase A chain (ldha) CSB-YP012832LON
CSB-EP012832LON
CSB-BP012832LON
CSB-MP012832LON
CSB-EP012832LON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor 2 (fusA), partial CSB-YP007434MRZ
CSB-EP007434MRZ
CSB-BP007434MRZ
CSB-MP007434MRZ
CSB-EP007434MRZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa 40S ribosomal protein S21 (rps-21) CSB-YP020399NHA
CSB-EP020399NHA
CSB-BP020399NHA
CSB-MP020399NHA
CSB-EP020399NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachysolen tannophilus Cytochrome c (CYC1) CSB-YP006328EQH
CSB-EP006328EQH
CSB-BP006328EQH
CSB-MP006328EQH
CSB-EP006328EQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune 40S ribosomal protein S26 (RPS26) CSB-YP020407SXR
CSB-EP020407SXR
CSB-BP020407SXR
CSB-MP020407SXR
CSB-EP020407SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans 60S ribosomal protein L32 (RPL32) CSB-YP020237CZF
CSB-EP020237CZF
CSB-BP020237CZF
CSB-MP020237CZF
CSB-EP020237CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Porphobilinogen deaminase (HEM3) CSB-YP010524CZD
CSB-EP010524CZD
CSB-BP010524CZD
CSB-MP010524CZD
CSB-EP010524CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S ribosomal protein L14 (rpl14) CSB-YP020142SXV
CSB-EP020142SXV
CSB-BP020142SXV
CSB-MP020142SXV
CSB-EP020142SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Midasin (mdn1), partial CSB-YP013628SXV
CSB-EP013628SXV
CSB-BP013628SXV
CSB-MP013628SXV
CSB-EP013628SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase III subunit rpc8 (rpc25) CSB-YP018351SXV
CSB-EP018351SXV
CSB-BP018351SXV
CSB-MP018351SXV
CSB-EP018351SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe COP9 signalosome complex subunit 1 (csn1) CSB-YP009858SXV
CSB-EP009858SXV
CSB-BP009858SXV
CSB-MP009858SXV
CSB-EP009858SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Argininosuccinate synthase (arg12) CSB-YP002234SXV
CSB-EP002234SXV
CSB-BP002234SXV
CSB-MP002234SXV
CSB-EP002234SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Importin subunit alpha-2 (imp1), partial CSB-YP012484SXV
CSB-EP012484SXV
CSB-BP012484SXV
CSB-MP012484SXV
CSB-EP012484SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pre-mRNA-splicing factor 18 (prp18) CSB-YP018760SXV
CSB-EP018760SXV
CSB-BP018760SXV
CSB-MP018760SXV
CSB-EP018760SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe MAU2 chromatid cohesion factor homolog (ssl3), partial CSB-YP012213SXV
CSB-EP012213SXV
CSB-BP012213SXV
CSB-MP012213SXV
CSB-EP012213SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe T-complex protein 1 subunit alpha (cct1), partial CSB-YP023320SXV
CSB-EP023320SXV
CSB-BP023320SXV
CSB-MP023320SXV
CSB-EP023320SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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