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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Burkholderia cenocepacia Argininosuccinate synthase (argG) CSB-YP002234BXQ
CSB-EP002234BXQ
CSB-BP002234BXQ
CSB-MP002234BXQ
CSB-EP002234BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Methionyl-tRNA formyltransferase (fmt) CSB-YP015150BXQ
CSB-EP015150BXQ
CSB-BP015150BXQ
CSB-MP015150BXQ
CSB-EP015150BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BXQ
CSB-EP005824BXQ
CSB-BP005824BXQ
CSB-MP005824BXQ
CSB-EP005824BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia tRNA dimethylallyltransferase (miaA) CSB-YP024529BXQ
CSB-EP024529BXQ
CSB-BP024529BXQ
CSB-MP024529BXQ
CSB-EP024529BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Peptide chain release factor 1 (prfA) CSB-YP015197BXQ
CSB-EP015197BXQ
CSB-BP015197BXQ
CSB-MP015197BXQ
CSB-EP015197BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia cenocepacia Glycine cleavage system H protein (gcvH) CSB-YP009335BXQ
CSB-EP009335BXQ
CSB-BP009335BXQ
CSB-MP009335BXQ
CSB-EP009335BXQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 ATP synthase subunit b (atpF), partial CSB-YP002358YAX1
CSB-EP002358YAX1
CSB-BP002358YAX1
CSB-MP002358YAX1
CSB-EP002358YAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 6-phosphofructokinase (pfkA) CSB-YP017822YAX
CSB-EP017822YAX
CSB-BP017822YAX
CSB-MP017822YAX
CSB-EP017822YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 S-adenosylmethionine decarboxylase proenzyme (speD) CSB-YP001658YAX
CSB-EP001658YAX
CSB-BP001658YAX
CSB-MP001658YAX
CSB-EP001658YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Peptide deformylase (def) CSB-YP017707YAX
CSB-EP017707YAX
CSB-BP017707YAX
CSB-MP017707YAX
CSB-EP017707YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:3 Elongation factor G (fusA), partial CSB-YP009373YAX
CSB-EP009373YAX
CSB-BP009373YAX
CSB-MP009373YAX
CSB-EP009373YAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Nucleoside diphosphate kinase (ndk) CSB-YP015889FGC
CSB-EP015889FGC
CSB-BP015889FGC
CSB-MP015889FGC
CSB-EP015889FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582FGC
CSB-EP009582FGC
CSB-BP009582FGC
CSB-MP009582FGC
CSB-EP009582FGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Agmatinase (speB) CSB-YP001447ENT
CSB-EP001447ENT
CSB-BP001447ENT
CSB-MP001447ENT
CSB-EP001447ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycine cleavage system H protein (gcvH) CSB-YP009335ENT
CSB-EP009335ENT
CSB-BP009335ENT
CSB-MP009335ENT
CSB-EP009335ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cysteine desulfurase (iscS) CSB-YP015769ENT
CSB-EP015769ENT
CSB-BP015769ENT
CSB-MP015769ENT
CSB-EP015769ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Peptide chain release factor 1 (prfA) CSB-YP015197ENT
CSB-EP015197ENT
CSB-BP015197ENT
CSB-MP015197ENT
CSB-EP015197ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Porphobilinogen deaminase (hemC) CSB-YP010524ENT
CSB-EP010524ENT
CSB-BP010524ENT
CSB-MP010524ENT
CSB-EP010524ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Xaa-Pro dipeptidase (pepQ) CSB-YP017784ENT
CSB-EP017784ENT
CSB-BP017784ENT
CSB-MP017784ENT
CSB-EP017784ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase subunit b (atpF), partial CSB-YP002358ENT1
CSB-EP002358ENT1
CSB-BP002358ENT1
CSB-MP002358ENT1
CSB-EP002358ENT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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