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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe T-complex protein 1 subunit zeta (cct6), partial CSB-YP004865SXV
CSB-EP004865SXV
CSB-BP004865SXV
CSB-MP004865SXV
CSB-EP004865SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Ribonuclease H2 subunit B (rnh202) CSB-YP019806SXV
CSB-EP019806SXV
CSB-BP019806SXV
CSB-MP019806SXV
CSB-EP019806SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Replication factor C subunit 5 (rfc5) CSB-YP019592SXV
CSB-EP019592SXV
CSB-BP019592SXV
CSB-MP019592SXV
CSB-EP019592SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA-directed RNA polymerase I subunit RPA12 (rpa12) CSB-YP027114SXV
CSB-EP027114SXV
CSB-BP027114SXV
CSB-MP027114SXV
CSB-EP027114SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Enhancer of mRNA-decapping protein 3 (edc3) CSB-YP007393SXV
CSB-EP007393SXV
CSB-BP007393SXV
CSB-MP007393SXV
CSB-EP007393SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Actin-related protein 2/3 complex subunit 4 (arcD) CSB-YP002130DKK
CSB-EP002130DKK
CSB-BP002130DKK
CSB-MP002130DKK
CSB-EP002130DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Alkyldihydroxyacetonephosphate synthase (eapA), partial CSB-YP001457DKK
CSB-EP001457DKK
CSB-BP001457DKK
CSB-MP001457DKK
CSB-EP001457DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkyldihydroxyacetonephosphate synthase, partial CSB-YP001457TQV
CSB-EP001457TQV
CSB-BP001457TQV
CSB-MP001457TQV
CSB-EP001457TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Giardia intestinalis Glucosamine-6-phosphate isomerase 2 (GPI2) CSB-YP009631GFW
CSB-EP009631GFW
CSB-BP009631GFW
CSB-MP009631GFW
CSB-EP009631GFW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dog C-X-C chemokine receptor type 2 (CXCR2), partial CSB-YP011673DO1
CSB-EP011673DO1
CSB-BP011673DO1
CSB-MP011673DO1
CSB-EP011673DO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithorhynchus anatinus Amelogenin (AMEL) CSB-YP001664OEX
CSB-EP001664OEX
CSB-BP001664OEX
CSB-MP001664OEX
CSB-EP001664OEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Suncus murinus Progonadoliberin-2 (GNRH2) CSB-YP009636FPS
CSB-EP009636FPS
CSB-BP009636FPS
CSB-MP009636FPS
CSB-EP009636FPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pygathrix bieti C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844FHN1
CSB-EP004844FHN1
CSB-BP004844FHN1
CSB-MP004844FHN1
CSB-EP004844FHN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhinopithecus avunculus C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844RKF1
CSB-EP004844RKF1
CSB-BP004844RKF1
CSB-MP004844RKF1
CSB-EP004844RKF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072EWO
CSB-EP015072EWO
CSB-BP015072EWO
CSB-MP015072EWO
CSB-EP015072EWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Connochaetes gnou Cytochrome b (MT-CYB), partial CSB-YP015075DVQ
CSB-EP015075DVQ
CSB-BP015075DVQ
CSB-MP015075DVQ
CSB-EP015075DVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Madoqua kirkii Cytochrome b (MT-CYB), partial CSB-YP015075MPL
CSB-EP015075MPL
CSB-BP015075MPL
CSB-MP015075MPL
CSB-EP015075MPL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eulemur macaco macaco Cytochrome b (MT-CYB), partial CSB-YP015075EPG
CSB-EP015075EPG
CSB-BP015075EPG
CSB-MP015075EPG
CSB-EP015075EPG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus ATP synthase protein 8 (MT-ATP8), partial CSB-YP015071RKI
CSB-EP015071RKI
CSB-BP015071RKI
CSB-MP015071RKI
CSB-EP015071RKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhipicephalus sanguineus NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080RKI1
CSB-EP015080RKI1
CSB-BP015080RKI1
CSB-MP015080RKI1
CSB-EP015080RKI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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