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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Meriones unguiculatus Beta-2 adrenergic receptor (ADRB2), partial CSB-YP001392MRA1
CSB-EP001392MRA1
CSB-BP001392MRA1
CSB-MP001392MRA1
CSB-EP001392MRA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Fragile X mental retardation syndrome-related protein 1 (FXR1), partial CSB-YP009087DXU
CSB-EP009087DXU
CSB-BP009087DXU
CSB-MP009087DXU
CSB-EP009087DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tetraodon fluviatilis Amyloid beta A4 protein (app), partial CSB-YP001950TLV1
CSB-EP001950TLV1
CSB-BP001950TLV1
CSB-MP001950TLV1
CSB-EP001950TLV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Gremlin-1 (grem1) CSB-YP009892XBE
CSB-EP009892XBE
CSB-BP009892XBE
CSB-MP009892XBE
CSB-EP009892XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes 40S ribosomal protein S3a (rps3a) CSB-YP020444OFH
CSB-EP020444OFH
CSB-BP020444OFH
CSB-MP020444OFH
CSB-EP020444OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Alpha-crystallin A chain (cryaa) CSB-YP006007OFH
CSB-EP006007OFH
CSB-BP006007OFH
CSB-MP006007OFH
CSB-EP006007OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cyberlindnera jadinii Squalene synthase (ERG9), partial CSB-YP008562EPX1
CSB-EP008562EPX1
CSB-BP008562EPX1
CSB-MP008562EPX1
CSB-EP008562EPX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uricase (SPCC1223.09) CSB-YP025648SXV
CSB-EP025648SXV
CSB-BP025648SXV
CSB-MP025648SXV
CSB-EP025648SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Coatomer subunit delta (ret2) CSB-YP001982SXV
CSB-EP001982SXV
CSB-BP001982SXV
CSB-MP001982SXV
CSB-EP001982SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA mismatch repair protein msh6 (msh6), partial CSB-YP015032SXV
CSB-EP015032SXV
CSB-BP015032SXV
CSB-MP015032SXV
CSB-EP015032SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe BTB/POZ domain-containing protein 2 (btb2) CSB-YP002846SXV
CSB-EP002846SXV
CSB-BP002846SXV
CSB-MP002846SXV
CSB-EP002846SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S acidic ribosomal protein P0 (rpp0) CSB-YP020336SXV
CSB-EP020336SXV
CSB-BP020336SXV
CSB-MP020336SXV
CSB-EP020336SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe BTB/POZ domain-containing protein 1 (btb1), partial CSB-YP002840SXV
CSB-EP002840SXV
CSB-BP002840SXV
CSB-MP002840SXV
CSB-EP002840SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Bax inhibitor 1 (bxi1), partial CSB-YP023632SXV1
CSB-EP023632SXV1
CSB-BP023632SXV1
CSB-MP023632SXV1
CSB-EP023632SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Exosome complex component rrp45 (rrp45) CSB-YP007897SXV
CSB-EP007897SXV
CSB-BP007897SXV
CSB-MP007897SXV
CSB-EP007897SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 60S ribosomal protein L12 (rpl1201) CSB-YP020116SXV
CSB-EP020116SXV
CSB-BP020116SXV
CSB-MP020116SXV
CSB-EP020116SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ribonuclease P protein subunit p40 (RPP40) CSB-YP020351HU
CSB-EP020351HU
CSB-BP020351HU
CSB-MP020351HU
CSB-EP020351HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytosolic carboxypeptidase 1 (ccpp-1), partial CSB-YP001464CXY
CSB-EP001464CXY
CSB-BP001464CXY
CSB-MP001464CXY
CSB-EP001464CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Regulator of nonsense transcripts 1 (smg-2), partial CSB-YP025650CXY
CSB-EP025650CXY
CSB-BP025650CXY
CSB-MP025650CXY
CSB-EP025650CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anopheles gambiae 60S ribosomal protein L7a (RpL7A) CSB-YP020303BZL
CSB-EP020303BZL
CSB-BP020303BZL
CSB-MP020303BZL
CSB-EP020303BZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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