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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. DNA polymerase III subunit beta (dnaN) CSB-YP300351SSQ
CSB-EP300351SSQ
CSB-BP300351SSQ
CSB-MP300351SSQ
CSB-EP300351SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative histidinol dehydrogenase 2 (hisD2) CSB-YP300353SSQ
CSB-EP300353SSQ
CSB-BP300353SSQ
CSB-MP300353SSQ
CSB-EP300353SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Lipoyl synthase 1 (lipA1) CSB-YP300354SSQ
CSB-EP300354SSQ
CSB-BP300354SSQ
CSB-MP300354SSQ
CSB-EP300354SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0284 protein sll1500 (sll1500) CSB-YP300355SSQ
CSB-EP300355SSQ
CSB-BP300355SSQ
CSB-MP300355SSQ
CSB-EP300355SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein ssr3122 (ssr3122) CSB-YP300356SSQ
CSB-EP300356SSQ
CSB-BP300356SSQ
CSB-MP300356SSQ
CSB-EP300356SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem II reaction center protein J (psbJ), partial CSB-YP300357SSQ
CSB-EP300357SSQ
CSB-BP300357SSQ
CSB-MP300357SSQ
CSB-EP300357SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Chromophore lyase CpcS/CpeS 2 (cpcS2) CSB-YP300358SSQ
CSB-EP300358SSQ
CSB-BP300358SSQ
CSB-MP300358SSQ
CSB-EP300358SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized isomerase slr1019 (slr1019) CSB-YP300359SSQ
CSB-EP300359SSQ
CSB-BP300359SSQ
CSB-MP300359SSQ
CSB-EP300359SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized thylakoid-associated protein sll0982 (sll0982) CSB-YP300360SSQ
CSB-EP300360SSQ
CSB-BP300360SSQ
CSB-MP300360SSQ
CSB-EP300360SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ferrous iron transport protein B homolog (feoB), partial CSB-YP300361SSQ
CSB-EP300361SSQ
CSB-BP300361SSQ
CSB-MP300361SSQ
CSB-EP300361SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative dihydroflavonol-4-reductase (dfrA) CSB-YP300362SSQ
CSB-EP300362SSQ
CSB-BP300362SSQ
CSB-MP300362SSQ
CSB-EP300362SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Thioredoxin-like protein slr1139 (slr1139) CSB-YP300363SSQ
CSB-EP300363SSQ
CSB-BP300363SSQ
CSB-MP300363SSQ
CSB-EP300363SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L25 (rplY) CSB-YP300364SSQ
CSB-EP300364SSQ
CSB-BP300364SSQ
CSB-MP300364SSQ
CSB-EP300364SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L17 (rplQ) CSB-YP300365SSQ
CSB-EP300365SSQ
CSB-BP300365SSQ
CSB-MP300365SSQ
CSB-EP300365SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L6 (rplF) CSB-YP300366SSQ
CSB-EP300366SSQ
CSB-BP300366SSQ
CSB-MP300366SSQ
CSB-EP300366SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L22 (rplV) CSB-YP300367SSQ
CSB-EP300367SSQ
CSB-BP300367SSQ
CSB-MP300367SSQ
CSB-EP300367SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Acetylglutamate kinase (argB) CSB-YP300368SSQ
CSB-EP300368SSQ
CSB-BP300368SSQ
CSB-MP300368SSQ
CSB-EP300368SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Chromosome partition protein Smc (smc), partial CSB-YP300369SSQ
CSB-EP300369SSQ
CSB-BP300369SSQ
CSB-MP300369SSQ
CSB-EP300369SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized RNA methyltransferase sll1967 (sll1967) CSB-YP300370SSQ
CSB-EP300370SSQ
CSB-BP300370SSQ
CSB-MP300370SSQ
CSB-EP300370SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glucose-6-phosphate 1-dehydrogenase (zwf), partial CSB-YP300371SSQ
CSB-EP300371SSQ
CSB-BP300371SSQ
CSB-MP300371SSQ
CSB-EP300371SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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