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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Long-chain-fatty-acid--CoA ligase (fadD) CSB-YP300208ENV
CSB-BP300208ENV
CSB-MP300208ENV
CSB-EP300208ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Cytochrome b6-f complex subunit 5 (petG), partial CSB-YP300209ZAX
CSB-EP300209ZAX
CSB-BP300209ZAX
CSB-MP300209ZAX
CSB-EP300209ZAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Feline immunodeficiency virus Probable Vpr-like protein CSB-YP300210FAM
CSB-EP300210FAM
CSB-BP300210FAM
CSB-MP300210FAM
CSB-EP300210FAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nepenthes distillatoria Aspartic proteinase nepenthesin-2 CSB-YP300211NCC
CSB-EP300211NCC
CSB-BP300211NCC
CSB-MP300211NCC
CSB-EP300211NCC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rotavirus C Intermediate capsid protein VP6 CSB-YP300212RHH
CSB-EP300212RHH
CSB-BP300212RHH
CSB-MP300212RHH
CSB-EP300212RHH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus imperialis Conotoxin Im11.2 CSB-YP300213DWA
CSB-EP300213DWA
CSB-BP300213DWA
CSB-MP300213DWA
CSB-EP300213DWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pepper mild mottle virus Capsid protein (CP) CSB-YP300214PFK
CSB-EP300214PFK
CSB-BP300214PFK
CSB-MP300214PFK
CSB-EP300214PFK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tomato bushy stunt virus RNA silencing suppressor p19 (ORF4) CSB-YP300215TGC
CSB-EP300215TGC
CSB-BP300215TGC
CSB-MP300215TGC
CSB-EP300215TGC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M13 Head virion protein G6P (VI), partial CSB-YP300216ECY1
CSB-EP300216ECY1
CSB-BP300216ECY1
CSB-MP300216ECY1
CSB-EP300216ECY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage f1 Tail virion protein G9P (IX), partial CSB-YP300217ECH
CSB-EP300217ECH
CSB-BP300217ECH
CSB-MP300217ECH
CSB-EP300217ECH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage f1 DNA-Binding protein G5P (V) CSB-YP300218ECH
CSB-EP300218ECH
CSB-BP300218ECH
CSB-MP300218ECH
CSB-EP300218ECH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pinus thunbergii Photosystem Q (B) protein, partial CSB-YP300219EVZ1
CSB-EP300219EVZ1
CSB-BP300219EVZ1
CSB-MP300219EVZ1
CSB-EP300219EVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudotsuga japonica Photosystem II reaction center protein I (psbI), partial CSB-YP300220PWL
CSB-EP300220PWL
CSB-BP300220PWL
CSB-MP300220PWL
CSB-EP300220PWL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solanum nigrum Photosystem Q (B) protein, partial CSB-YP300221SIR1
CSB-EP300221SIR1
CSB-BP300221SIR1
CSB-MP300221SIR1
CSB-EP300221SIR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Larix occidentalis Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP300222LCH
CSB-EP300222LCH
CSB-BP300222LCH
CSB-MP300222LCH
CSB-EP300222LCH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus arboreus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP300223LJS
CSB-EP300223LJS
CSB-BP300223LJS
CSB-MP300223LJS
CSB-EP300223LJS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus luteus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP300224LRK
CSB-EP300224LRK
CSB-BP300224LRK
CSB-MP300224LRK
CSB-EP300224LRK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus pilosus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP300225LKI
CSB-EP300225LKI
CSB-BP300225LKI
CSB-MP300225LKI
CSB-EP300225LKI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Nucleoprotein (N) CSB-YP300226ARK
CSB-EP300226ARK
CSB-BP300226ARK
CSB-EP300226ARK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Membrane protein (M), partial CSB-YP300227ART1
CSB-EP300227ART1
CSB-BP300227ART1
CSB-MP300227ART1
CSB-EP300227ART1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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