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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli Fructose-like permease IIC component 1 (fryC), partial CSB-YP302059ENV
CSB-EP302059ENV
CSB-BP302059ENV
CSB-MP302059ENV
CSB-EP302059ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative 3-hydroxyphenylpropionic acid transporter (mhpT), partial CSB-YP302060ENV
CSB-EP302060ENV
CSB-BP302060ENV
CSB-MP302060ENV
CSB-EP302060ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ydcN (ydcN) CSB-YP302061ENV
CSB-EP302061ENV
CSB-BP302061ENV
CSB-MP302061ENV
CSB-EP302061ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfdK (yfdK) CSB-YP302062ENV
CSB-EP302062ENV
CSB-BP302062ENV
CSB-MP302062ENV
CSB-EP302062ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase-4 component I (hyfI) CSB-YP302063ENV
CSB-EP302063ENV
CSB-BP302063ENV
CSB-MP302063ENV
CSB-EP302063ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ybcK (ybcK) CSB-YP302064ENV
CSB-EP302064ENV
CSB-BP302064ENV
CSB-MP302064ENV
CSB-EP302064ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane ABC transporter permease protein ycjP (ycjP), partial CSB-YP302065ENV
CSB-EP302065ENV
CSB-BP302065ENV
CSB-MP302065ENV
CSB-EP302065ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane transport protein yajR (yajR), partial CSB-YP302066ENV
CSB-EP302066ENV
CSB-BP302066ENV
CSB-MP302066ENV
CSB-EP302066ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized oxidoreductase YajO (yajO) CSB-YP302067ENV
CSB-EP302067ENV
CSB-BP302067ENV
CSB-MP302067ENV
CSB-EP302067ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yfcI (yfcI) CSB-YP302068ENV
CSB-EP302068ENV
CSB-BP302068ENV
CSB-MP302068ENV
CSB-EP302068ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized fimbrial-like protein ydeS (ydeS) CSB-YP302069ENV
CSB-EP302069ENV
CSB-BP302069ENV
CSB-MP302069ENV
CSB-EP302069ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Methionine aminotransferase (ybdL) CSB-YP302070ENV
CSB-EP302070ENV
CSB-BP302070ENV
CSB-MP302070ENV
CSB-EP302070ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Pyrimidine-nucleoside phosphorylase (pdp) CSB-YP302072GFM
CSB-EP302072GFM
CSB-BP302072GFM
CSB-MP302072GFM
CSB-EP302072GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans Chaperone protein DnaJ (dnaJ) CSB-YP302073AYK
CSB-EP302073AYK
CSB-BP302073AYK
CSB-MP302073AYK
CSB-EP302073AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Carbamoyl-phosphate synthase pyrimidine-specific small chain (pyrAA) CSB-YP302074LMS
CSB-EP302074LMS
CSB-BP302074LMS
CSB-MP302074LMS
CSB-EP302074LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable cation-transporting ATPase V (ctpV), partial CSB-YP302075MVZ
CSB-EP302075MVZ
CSB-BP302075MVZ
CSB-MP302075MVZ
CSB-EP302075MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus abyssi Aspartate carbamoyltransferase (pyrB) CSB-YP302076FHV
CSB-EP302076FHV
CSB-BP302076FHV
CSB-MP302076FHV
CSB-EP302076FHV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus kodakaraensis DNA polymerase (pol) CSB-YP302077FHY
CSB-EP302077FHY
CSB-BP302077FHY
CSB-MP302077FHY
CSB-EP302077FHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Superoxide dismutase [Fe] (sodB) CSB-YP302078SSQ
CSB-EP302078SSQ
CSB-BP302078SSQ
CSB-MP302078SSQ
CSB-EP302078SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Pyruvate kinase I (pykF) CSB-YP302079SXB
CSB-EP302079SXB
CSB-BP302079SXB
CSB-MP302079SXB
CSB-EP302079SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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