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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Malate synthase (aceB), partial CSB-YP300618SNL
CSB-EP300618SNL
CSB-BP300618SNL
CSB-MP300618SNL
CSB-EP300618SNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate--tRNA ligase (gltX) CSB-YP300619FLV
CSB-EP300619FLV
CSB-BP300619FLV
CSB-MP300619FLV
CSB-EP300619FLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae S-adenosylmethionine synthase (metK) CSB-YP300620MLW
CSB-EP300620MLW
CSB-BP300620MLW
CSB-MP300620MLW
CSB-EP300620MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L33 1 (rpmG1) CSB-YP300621MLW
CSB-EP300621MLW
CSB-BP300621MLW
CSB-MP300621MLW
CSB-EP300621MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae RNA polymerase sigma factor rpoD (rpoD) CSB-YP300622MLW
CSB-EP300622MLW
CSB-BP300622MLW
CSB-MP300622MLW
CSB-EP300622MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L1 (rplA) CSB-YP300623MLW
CSB-EP300623MLW
CSB-BP300623MLW
CSB-MP300623MLW
CSB-EP300623MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vibrio cholerae serotype O1 Protein translocase subunit SecY (secY), partial CSB-YP300624VEX
CSB-EP300624VEX
CSB-BP300624VEX
CSB-MP300624VEX
CSB-EP300624VEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus oryzae Tannase (AO090103000074), partial CSB-YP300625DPA
CSB-EP300625DPA
CSB-BP300625DPA
CSB-MP300625DPA
CSB-EP300625DPA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Iron transport multicopper oxidase FET3 (FET3), partial CSB-YP300626CZF1
CSB-EP300626CZF1
CSB-BP300626CZF1
CSB-MP300626CZF1
CSB-EP300626CZF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Chitin synthase D (chsD), partial CSB-YP300627EKF
CSB-EP300627EKF
CSB-BP300627EKF
CSB-MP300627EKF
CSB-EP300627EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Dimethyladenosine transferase (DIM1) CSB-YP300628KBK
CSB-EP300628KBK
CSB-BP300628KBK
CSB-MP300628KBK
CSB-EP300628KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Podospora anserina Laccase-2 (LAC2), partial CSB-YP300629EXI
CSB-EP300629EXI
CSB-BP300629EXI
CSB-MP300629EXI
CSB-EP300629EXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Probable transcriptional regulatory protein YebC (yebC) CSB-YP300630SXB
CSB-EP300630SXB
CSB-BP300630SXB
CSB-MP300630SXB
CSB-EP300630SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable transcriptional regulatory protein SPy_0316/M5005_Spy0269 (SPy_0316, M5005_Spy0269) CSB-YP300631SMT
CSB-EP300631SMT
CSB-BP300631SMT
CSB-MP300631SMT
CSB-EP300631SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a Putative phosphotransferase lin1494 (lin1494) CSB-YP300632LGU
CSB-EP300632LGU
CSB-BP300632LGU
CSB-MP300632LGU
CSB-EP300632LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative diacyglycerol O-acyltransferase Rv0895/MT0919 (Rv0895, MT0919), partial CSB-YP300633MVZ
CSB-EP300633MVZ
CSB-BP300633MVZ
CSB-MP300633MVZ
CSB-EP300633MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative diacyglycerol O-acyltransferase Mb3761c (Mb3761c) CSB-YP300634MVH
CSB-EP300634MVH
CSB-BP300634MVH
CSB-MP300634MVH
CSB-EP300634MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0098 protein Mb1945c (Mb1945c) CSB-YP300635MVH
CSB-EP300635MVH
CSB-BP300635MVH
CSB-MP300635MVH
CSB-EP300635MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Epimerase family protein Rv2216/MT2273 (Rv2216, MT2273) CSB-YP300636MVZ
CSB-EP300636MVZ
CSB-BP300636MVZ
CSB-MP300636MVZ
CSB-EP300636MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable ribonuclease VapC42 (vapC42) CSB-YP300637MVZ
CSB-EP300637MVZ
CSB-BP300637MVZ
CSB-MP300637MVZ
CSB-EP300637MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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