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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acipenser stellatus Protamine-B CSB-YP302425ACB
CSB-EP302425ACB
CSB-BP302425ACB
CSB-MP302425ACB
CSB-EP302425ACB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Protamine-2B CSB-YP302426OEI
CSB-EP302426OEI
CSB-BP302426OEI
CSB-MP302426OEI
CSB-EP302426OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria aurea Aurein-3.2 CSB-YP302427LHE
CSB-EP302427LHE
CSB-BP302427LHE
CSB-MP302427LHE
CSB-EP302427LHE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Litoria splendida Splendipherin CSB-YP302428LHS
CSB-EP302428LHS
CSB-BP302428LHS
CSB-MP302428LHS
CSB-EP302428LHS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Antirrhinum majus Translation initiation factor IF-1, chloroplastic (infA) CSB-YP302429DNE
CSB-EP302429DNE
CSB-BP302429DNE
CSB-MP302429DNE
CSB-EP302429DNE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 60 kDa chaperonin (groL), partial CSB-YP302430LNY
CSB-BP302430LNY
CSB-MP302430LNY
CSB-EP302430LNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisaster ochraceus Histone H3, embryonic CSB-YP302431PKW
CSB-EP302431PKW
CSB-BP302431PKW
CSB-MP302431PKW
CSB-EP302431PKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus kisutch Peptide YY-like CSB-YP302432OBH
CSB-EP302432OBH
CSB-BP302432OBH
CSB-MP302432OBH
CSB-EP302432OBH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanine nucleotide-binding protein subunit beta-like protein CSB-YP302433TQX
CSB-EP302433TQX
CSB-BP302433TQX
CSB-MP302433TQX
CSB-EP302433TQX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus nerka Progonadoliberin-3 (gnrh3) CSB-YP302434OBI
CSB-EP302434OBI
CSB-BP302434OBI
CSB-MP302434OBI
CSB-EP302434OBI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anguilla anguilla Somatostatin-1 (sst1) CSB-YP302435BZF
CSB-EP302435BZF
CSB-BP302435BZF
CSB-MP302435BZF
CSB-EP302435BZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macrocallista nimbosa FMRFamide CSB-YP302436MAH
CSB-EP302436MAH
CSB-BP302436MAH
CSB-MP302436MAH
CSB-EP302436MAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 3-alpha- (or 20-beta)-hydroxysteroid dehydrogenase CSB-YP302437MVH
CSB-EP302437MVH
CSB-BP302437MVH
CSB-MP302437MVH
CSB-EP302437MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage S13 C protein (C) CSB-YP302438EDS
CSB-EP302438EDS
CSB-BP302438EDS
CSB-MP302438EDS
CSB-EP302438EDS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Inhibitor of apoptosis protein (BA71V-032) CSB-YP302439AEJ
CSB-EP302439AEJ
CSB-BP302439AEJ
CSB-MP302439AEJ
CSB-EP302439AEJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mumps virus Small hydrophobic protein (SH), partial CSB-YP302440MJE
CSB-EP302440MJE
CSB-BP302440MJE
CSB-MP302440MJE
CSB-EP302440MJE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c, chloroplastic (atpH), partial CSB-YP302441LQQ1
CSB-EP302441LQQ1
CSB-BP302441LQQ1
CSB-MP302441LQQ1
CSB-EP302441LQQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anthopleura elegantissima Morphogenetic neuropeptide CSB-YP302442DNA
CSB-EP302442DNA
CSB-BP302442DNA
CSB-MP302442DNA
CSB-EP302442DNA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S18 1 (rpsR1) CSB-YP302443MVH
CSB-EP302443MVH
CSB-BP302443MVH
CSB-MP302443MVH
CSB-EP302443MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Influenza B virus Non-structural protein 1 (NS) CSB-YP302444IIQ
CSB-EP302444IIQ
CSB-BP302444IIQ
CSB-MP302444IIQ
CSB-EP302444IIQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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