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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Phosphate import ATP-binding protein PstB 3 (pstB3) CSB-YP301023SSQ
CSB-EP301023SSQ
CSB-BP301023SSQ
CSB-MP301023SSQ
CSB-EP301023SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein ssr3402 (ssr3402) CSB-YP301025SSQ
CSB-EP301025SSQ
CSB-BP301025SSQ
CSB-MP301025SSQ
CSB-EP301025SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. (3R)-hydroxymyristoyl-[acyl-carrier-protein] dehydratase CSB-YP301026SSQ
CSB-EP301026SSQ
CSB-BP301026SSQ
CSB-MP301026SSQ
CSB-EP301026SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Potassium-transporting ATPase C chain (kdpC), partial CSB-YP301027SSQ1
CSB-EP301027SSQ1
CSB-BP301027SSQ1
CSB-MP301027SSQ1
CSB-EP301027SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr0269 (slr0269), partial CSB-YP301028SSQ1
CSB-EP301028SSQ1
CSB-BP301028SSQ1
CSB-MP301028SSQ1
CSB-EP301028SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glycerol-3-phosphate acyltransferase (plsY), partial CSB-YP301029SSQ1
CSB-EP301029SSQ1
CSB-BP301029SSQ1
CSB-MP301029SSQ1
CSB-EP301029SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable inorganic polyphosphate/ATP-NAD kinase 2 (ppnK2) CSB-YP301030SSQ
CSB-EP301030SSQ
CSB-BP301030SSQ
CSB-MP301030SSQ
CSB-EP301030SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Release factor glutamine methyltransferase (prmC) CSB-YP301031SSQ
CSB-EP301031SSQ
CSB-BP301031SSQ
CSB-MP301031SSQ
CSB-EP301031SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem I assembly protein ycf3 (ycf3) CSB-YP301032SSQ
CSB-EP301032SSQ
CSB-BP301032SSQ
CSB-MP301032SSQ
CSB-EP301032SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Serine acetyltransferase (cysE) CSB-YP301033SSQ
CSB-EP301033SSQ
CSB-BP301033SSQ
CSB-MP301033SSQ
CSB-EP301033SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. GTPase Der (der) CSB-YP301034SSQ
CSB-EP301034SSQ
CSB-BP301034SSQ
CSB-MP301034SSQ
CSB-EP301034SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase 2 (acsF2) CSB-YP301035SSQ
CSB-EP301035SSQ
CSB-BP301035SSQ
CSB-MP301035SSQ
CSB-EP301035SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Membrane protein insertase YidC (yidC), partial CSB-YP301036SSQ1
CSB-EP301036SSQ1
CSB-BP301036SSQ1
CSB-MP301036SSQ1
CSB-EP301036SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Gamma-glutamyltranspeptidase (ggt) CSB-YP301037SSQ
CSB-EP301037SSQ
CSB-BP301037SSQ
CSB-MP301037SSQ
CSB-EP301037SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit B CSB-YP301038SSQ
CSB-EP301038SSQ
CSB-BP301038SSQ
CSB-MP301038SSQ
CSB-EP301038SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S12 (rpsL) CSB-YP301039SSQ
CSB-EP301039SSQ
CSB-BP301039SSQ
CSB-MP301039SSQ
CSB-EP301039SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glucosylglycerol-phosphate synthase (ggpS) CSB-YP301040SSQ
CSB-EP301040SSQ
CSB-BP301040SSQ
CSB-MP301040SSQ
CSB-EP301040SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized AIPM/Hcit synthase family transferase sll1564 (sll1564), partial CSB-YP301041SSQ
CSB-EP301041SSQ
CSB-BP301041SSQ
CSB-MP301041SSQ
CSB-EP301041SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC) CSB-YP301042SSQ
CSB-EP301042SSQ
CSB-BP301042SSQ
CSB-MP301042SSQ
CSB-EP301042SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Mini-ribonuclease 3 (mrnC) CSB-YP301043SSQ
CSB-EP301043SSQ
CSB-BP301043SSQ
CSB-MP301043SSQ
CSB-EP301043SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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