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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermotoga maritima DNA mismatch repair protein MutS (mutS), partial CSB-YP301064TNJ
CSB-EP301064TNJ
CSB-BP301064TNJ
CSB-MP301064TNJ
CSB-EP301064TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Gamma-glutamyl phosphate reductase (proA) CSB-YP301065TPR
CSB-EP301065TPR
CSB-BP301065TPR
CSB-MP301065TPR
CSB-EP301065TPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable isochorismatase (venB) CSB-YP301066VFI
CSB-EP301066VFI
CSB-BP301066VFI
CSB-MP301066VFI
CSB-EP301066VFI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tellurium resistance protein TerX (terX) CSB-YP301067SYN
CSB-EP301067SYN
CSB-BP301067SYN
CSB-MP301067SYN
CSB-EP301067SYN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative mgpC-like protein MPN_150 (MPN_150) CSB-YP301068MLW
CSB-EP301068MLW
CSB-BP301068MLW
CSB-MP301068MLW
CSB-EP301068MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae SsrA-binding protein (smpB) CSB-YP301069MLW
CSB-EP301069MLW
CSB-BP301069MLW
CSB-MP301069MLW
CSB-EP301069MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Phosphomannomutase (manB), partial CSB-YP301070MLW
CSB-EP301070MLW
CSB-BP301070MLW
CSB-MP301070MLW
CSB-EP301070MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Spermidine/putrescine transport system permease protein PotC homolog (potC), partial CSB-YP301071MLW1
CSB-EP301071MLW1
CSB-BP301071MLW1
CSB-MP301071MLW1
CSB-EP301071MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_048 (MPN_048), partial CSB-YP301072MLW
CSB-EP301072MLW
CSB-BP301072MLW
CSB-MP301072MLW
CSB-EP301072MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_041 (MPN_041) CSB-YP301073MLW
CSB-EP301073MLW
CSB-BP301073MLW
CSB-MP301073MLW
CSB-EP301073MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_036 (MPN_036), partial CSB-YP301074MLW
CSB-EP301074MLW
CSB-BP301074MLW
CSB-MP301074MLW
CSB-EP301074MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Elongation factor P (efp) CSB-YP301075MLW
CSB-EP301075MLW
CSB-BP301075MLW
CSB-MP301075MLW
CSB-EP301075MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized ATP-dependent helicase MPN_020 (MPN_020), partial CSB-YP301076MLW
CSB-EP301076MLW
CSB-BP301076MLW
CSB-MP301076MLW
CSB-EP301076MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0134 protein MPN_010 (MPN_010) CSB-YP301077MLW
CSB-EP301077MLW
CSB-BP301077MLW
CSB-MP301077MLW
CSB-EP301077MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Membrane protein insertase YidC (yidC), partial CSB-YP301078MLW
CSB-EP301078MLW
CSB-BP301078MLW
CSB-MP301078MLW
CSB-EP301078MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG459 homolog (MPN_673) CSB-YP301079MLW
CSB-EP301079MLW
CSB-BP301079MLW
CSB-MP301079MLW
CSB-EP301079MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG447 homolog (MPN_661), partial CSB-YP301080MLW
CSB-EP301080MLW
CSB-BP301080MLW
CSB-MP301080MLW
CSB-EP301080MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0134 protein MPN_655 (MPN_655) CSB-YP301081MLW
CSB-EP301081MLW
CSB-BP301081MLW
CSB-MP301081MLW
CSB-EP301081MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_143 (MPN_143) CSB-YP301082MLW
CSB-EP301082MLW
CSB-BP301082MLW
CSB-MP301082MLW
CSB-EP301082MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MG439 homolog 1 (MPN_647) CSB-YP301083MLW
CSB-EP301083MLW
CSB-BP301083MLW
CSB-MP301083MLW
CSB-EP301083MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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