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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Haemophilus influenzae Mu-like prophage FluMu protein gp16 (HI_1488) CSB-YP301772HTA
CSB-EP301772HTA
CSB-BP301772HTA
CSB-MP301772HTA
CSB-EP301772HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus influenzae RutC family protein HI_1627 (HI_1627) CSB-YP301773HTA
CSB-EP301773HTA
CSB-BP301773HTA
CSB-MP301773HTA
CSB-EP301773HTA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori ATP-dependent zinc metalloprotease FtsH (ftsH), partial CSB-YP301774HUV1
CSB-EP301774HUV1
CSB-BP301774HUV1
CSB-MP301774HUV1
CSB-EP301774HUV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA primase (dnaG), partial CSB-YP301775LNY
CSB-EP301775LNY
CSB-BP301775LNY
CSB-MP301775LNY
CSB-EP301775LNY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum Chaperone protein DnaJ (dnaJ) CSB-YP301776MLJ
CSB-EP301776MLJ
CSB-BP301776MLJ
CSB-MP301776MLJ
CSB-EP301776MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acyl-CoA dehydrogenase fadE12 (fadE12) CSB-YP301777MVZ
CSB-EP301777MVZ
CSB-BP301777MVZ
CSB-MP301777MVZ
CSB-EP301777MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis PP2C-family Ser/Thr phosphatase (pstP), partial CSB-YP301778GKB
CSB-EP301778GKB
CSB-BP301778GKB
CSB-MP301778GKB
CSB-EP301778GKB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant MFS-type drug efflux transporter P55 (Rv1410c, MT1454), partial CSB-YP301779MVZ
CSB-EP301779MVZ
CSB-BP301779MVZ
CSB-MP301779MVZ
CSB-EP301779MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Replicative DNA helicase (dnaB), partial CSB-YP301780MVZ
CSB-EP301780MVZ
CSB-BP301780MVZ
CSB-MP301780MVZ
CSB-EP301780MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfite reductase [ferredoxin] (sir), partial CSB-YP301781MVZ
CSB-EP301781MVZ
CSB-BP301781MVZ
CSB-MP301781MVZ
CSB-EP301781MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv1510/MT1558/MT1560 (Rv1510, MT1558/MT1560), partial CSB-YP301782MVZ
CSB-EP301782MVZ
CSB-BP301782MVZ
CSB-MP301782MVZ
CSB-EP301782MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized oxidoreductase Rv0769/MT0793 (Rv0769, MT0793) CSB-YP301783MVZ
CSB-EP301783MVZ
CSB-BP301783MVZ
CSB-MP301783MVZ
CSB-EP301783MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant RNA-splicing ligase RtcB (rtcB) CSB-YP301784MVZ
CSB-EP301784MVZ
CSB-BP301784MVZ
CSB-MP301784MVZ
CSB-EP301784MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv2676c/MT2750 (Rv2676c, MT2750) CSB-YP301785MVZ
CSB-EP301785MVZ
CSB-BP301785MVZ
CSB-MP301785MVZ
CSB-EP301785MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized membrane protein Rv1735c/MT1776 (Rv1735c, MT1776), partial CSB-YP301786MVZ1
CSB-EP301786MVZ1
CSB-BP301786MVZ1
CSB-MP301786MVZ1
CSB-EP301786MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isopentenyl-diphosphate Delta-isomerase (idi) CSB-YP301787MVZ
CSB-EP301787MVZ
CSB-BP301787MVZ
CSB-MP301787MVZ
CSB-EP301787MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-CoA decarbonylase/synthase complex subunit epsilon 1 (cdhB1) CSB-YP301788MSO
CSB-EP301788MSO
CSB-BP301788MSO
CSB-MP301788MSO
CSB-EP301788MSO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized protein Rv3776 (Rv3776), partial CSB-YP301789MVZ
CSB-EP301789MVZ
CSB-BP301789MVZ
CSB-MP301789MVZ
CSB-EP301789MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA gyrase subunit A (gyrA) CSB-YP301790MWB
CSB-EP301790MWB
CSB-BP301790MWB
CSB-MP301790MWB
CSB-EP301790MWB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Imidazole glycerol phosphate synthase subunit HisH 2 (hisH2) CSB-YP301791EZX
CSB-EP301791EZX
CSB-BP301791EZX
CSB-MP301791EZX
CSB-EP301791EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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