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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Enterobacteria phage phiX174 External scaffolding protein D (D) CSB-YP301670EDN
CSB-EP301670EDN
CSB-BP301670EDN
CSB-MP301670EDN
CSB-EP301670EDN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Conus miles Conotoxin Mi11.1 CSB-YP301671DWE
CSB-EP301671DWE
CSB-BP301671DWE
CSB-MP301671DWE
CSB-EP301671DWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tobacco mosaic virus Capsid protein (CP) CSB-YP301672TFE
CSB-BP301672TFE
CSB-MP301672TFE
CSB-EP301672TFE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cucumber green mottle mosaic virus Replicase large subunit CSB-YP301673CTU
CSB-EP301673CTU
CSB-BP301673CTU
CSB-MP301673CTU
CSB-EP301673CTU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage fd Head virion protein G6P (VI), partial CSB-YP301674ECJ1
CSB-EP301674ECJ1
CSB-BP301674ECJ1
CSB-MP301674ECJ1
CSB-EP301674ECJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M13 Tail virion protein G9P (IX), partial CSB-YP301675ECY
CSB-BP301675ECY
CSB-MP301675ECY
CSB-EP301675ECY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage M13 Replication-associated protein G2P (II) CSB-YP301676ECY
CSB-EP301676ECY
CSB-BP301676ECY
CSB-MP301676ECY
CSB-EP301676ECY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secale cereale Photosystem II reaction center protein H (psbH), partial CSB-YP301677STH1
CSB-EP301677STH1
CSB-BP301677STH1
CSB-MP301677STH1
CSB-EP301677STH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nicotiana debneyi Photosystem Q (B) protein, partial CSB-YP301678NDI1
CSB-EP301678NDI1
CSB-BP301678NDI1
CSB-MP301678NDI1
CSB-EP301678NDI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Euphorbia characias Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP301679EIE
CSB-EP301679EIE
CSB-BP301679EIE
CSB-MP301679EIE
CSB-EP301679EIE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus albescens Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP301680LJO
CSB-EP301680LJO
CSB-BP301680LJO
CSB-MP301680LJO
CSB-EP301680LJO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus hispanicus Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP301681LJZ
CSB-EP301681LJZ
CSB-BP301681LJZ
CSB-MP301681LJZ
CSB-EP301681LJZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lupinus princei Ribulose bisphosphate carboxylase large chain (rbcL) CSB-YP301682LKK
CSB-EP301682LKK
CSB-BP301682LKK
CSB-MP301682LKK
CSB-EP301682LKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Nucleoprotein (N) CSB-YP301683ARM
CSB-EP301683ARM
CSB-BP301683ARM
CSB-MP301683ARM
CSB-EP301683ARM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avian infectious bronchitis virus Membrane protein (M), partial CSB-YP301684ARR1
CSB-EP301684ARR1
CSB-BP301684ARR1
CSB-MP301684ARR1
CSB-EP301684ARR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porcine respiratory coronavirus Envelope small membrane protein (E), partial CSB-YP301685PQD1
CSB-EP301685PQD1
CSB-BP301685PQD1
CSB-MP301685PQD1
CSB-EP301685PQD1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hepatitis delta virus genotype II Small delta antigen CSB-YP301686HFV
CSB-EP301686HFV
CSB-BP301686HFV
CSB-MP301686HFV
CSB-EP301686HFV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemophilus phage S2 Uncharacterized 9.7 kDa protein in cox-rep intergenic region CSB-YP301687HAO
CSB-EP301687HAO
CSB-BP301687HAO
CSB-MP301687HAO
CSB-EP301687HAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP301688AOA
CSB-EP301688AOA
CSB-BP301688AOA
CSB-MP301688AOA
CSB-EP301688AOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Avenella flexuosa 30S ribosomal protein S4, chloroplastic (rps4) CSB-YP301689ARC
CSB-EP301689ARC
CSB-BP301689ARC
CSB-MP301689ARC
CSB-EP301689ARC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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