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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neurospora crassa Endoplasmic reticulum chaperone BiP (grp78), partial CSB-YP303569NHA
CSB-EP303569NHA
CSB-BP303569NHA
CSB-MP303569NHA
CSB-EP303569NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Transcriptional repressor rco-1 (rco-1), partial CSB-YP303570NHA
CSB-EP303570NHA
CSB-BP303570NHA
CSB-MP303570NHA
CSB-EP303570NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Fusarium solani subsp. pisi Dynein heavy chain, cytoplasmic (DHC1), partial CSB-YP303571FDP
CSB-EP303571FDP
CSB-BP303571FDP
CSB-MP303571FDP
CSB-EP303571FDP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pachysolen tannophilus NAD (P)H-dependent D-xylose reductase CSB-YP303572EQH
CSB-EP303572EQH
CSB-BP303572EQH
CSB-MP303572EQH
CSB-EP303572EQH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Vesicle transport v-SNARE protein vti1 (vti1), partial CSB-YP303574SXV
CSB-EP303574SXV
CSB-BP303574SXV
CSB-MP303574SXV
CSB-EP303574SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pre-mRNA-splicing factor cwc15 (cwf15) CSB-YP303575SXV
CSB-EP303575SXV
CSB-BP303575SXV
CSB-MP303575SXV
CSB-EP303575SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 6-phosphogluconate dehydrogenase, decarboxylating (SPBC660.16) CSB-YP303576SXV
CSB-EP303576SXV
CSB-BP303576SXV
CSB-MP303576SXV
CSB-EP303576SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Adenosine kinase (ado1) CSB-YP303577SXV
CSB-EP303577SXV
CSB-BP303577SXV
CSB-MP303577SXV
CSB-EP303577SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Nucleoporin nup186 (nup186), partial CSB-YP303578SXV
CSB-EP303578SXV
CSB-BP303578SXV
CSB-MP303578SXV
CSB-EP303578SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Trehalose-phosphatase (tpp1), partial CSB-YP303579SXV
CSB-EP303579SXV
CSB-BP303579SXV
CSB-MP303579SXV
CSB-EP303579SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pheromone receptor transcription activator (map1) CSB-YP303580SXV
CSB-EP303580SXV
CSB-BP303580SXV
CSB-MP303580SXV
CSB-EP303580SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces paradoxus Meiotic recombination protein REC114 (REC114) CSB-YP303581SAI
CSB-EP303581SAI
CSB-BP303581SAI
CSB-MP303581SAI
CSB-EP303581SAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica pH-response transcription factor pacC/RIM101 (RIM101), partial CSB-YP303582YAP
CSB-EP303582YAP
CSB-BP303582YAP
CSB-MP303582YAP
CSB-EP303582YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcriptional activator hap5 (hap5) CSB-YP303583SXV
CSB-EP303583SXV
CSB-BP303583SXV
CSB-MP303583SXV
CSB-EP303583SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S19-B (rps19b) CSB-YP303584SXV
CSB-EP303584SXV
CSB-BP303584SXV
CSB-MP303584SXV
CSB-EP303584SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe ATP-dependent helicase rhp16 (rhp16), partial CSB-YP303585SXV
CSB-EP303585SXV
CSB-BP303585SXV
CSB-MP303585SXV
CSB-EP303585SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe DNA damage tolerance protein rad31 (rad31) CSB-YP303586SXV
CSB-EP303586SXV
CSB-BP303586SXV
CSB-MP303586SXV
CSB-EP303586SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trametes versicolor Pyranose 2-oxidase (P2OX), partial CSB-YP303587TPK
CSB-EP303587TPK
CSB-BP303587TPK
CSB-MP303587TPK
CSB-EP303587TPK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Cleavage and polyadenylation specificity factor subunit 3 (CPSF3), partial CSB-YP303589BO
CSB-EP303589BO
CSB-BP303589BO
CSB-MP303589BO
CSB-EP303589BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Low affinity immunoglobulin gamma Fc region receptor III (FCGR3), partial CSB-YP303590BO
CSB-EP303590BO
CSB-BP303590BO
CSB-MP303590BO
CSB-EP303590BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產(chǎn)生大量蛋白質、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產(chǎn)生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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