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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. UDP-N-acetylmuramate--L-alanine ligase (murC), partial CSB-YP301876SSQ
CSB-EP301876SSQ
CSB-BP301876SSQ
CSB-MP301876SSQ
CSB-EP301876SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase CSB-YP301877SSQ
CSB-EP301877SSQ
CSB-BP301877SSQ
CSB-MP301877SSQ
CSB-EP301877SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized WD repeat-containing protein sll1491 (sll1491) CSB-YP301878SSQ
CSB-EP301878SSQ
CSB-BP301878SSQ
CSB-MP301878SSQ
CSB-EP301878SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0150 protein ssr1258 (ssr1258) CSB-YP301879SSQ
CSB-EP301879SSQ
CSB-BP301879SSQ
CSB-MP301879SSQ
CSB-EP301879SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Diaminopimelate epimerase (dapF) CSB-YP301880SSQ
CSB-EP301880SSQ
CSB-BP301880SSQ
CSB-MP301880SSQ
CSB-EP301880SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Histidine biosynthesis bifunctional protein HisIE (hisI) CSB-YP301882SSQ
CSB-EP301882SSQ
CSB-BP301882SSQ
CSB-MP301882SSQ
CSB-EP301882SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0175 protein ssl1255 (ssl1255) CSB-YP301883SSQ
CSB-EP301883SSQ
CSB-BP301883SSQ
CSB-MP301883SSQ
CSB-EP301883SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Secreted effector protein sptP (sptP), partial CSB-YP301884SWW
CSB-EP301884SWW
CSB-BP301884SWW
CSB-MP301884SWW
CSB-EP301884SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Secretion system apparatus protein ssaO (ssaO) CSB-YP301885SXB
CSB-EP301885SXB
CSB-BP301885SXB
CSB-MP301885SXB
CSB-EP301885SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP301886SXB
CSB-EP301886SXB
CSB-BP301886SXB
CSB-MP301886SXB
CSB-EP301886SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DNA polymerase (pol) CSB-YP301888TDF
CSB-EP301888TDF
CSB-BP301888TDF
CSB-MP301888TDF
CSB-EP301888TDF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum Flagellar biosynthetic protein fliP (fliP), partial CSB-YP301889TPR1
CSB-EP301889TPR1
CSB-BP301889TPR1
CSB-MP301889TPR1
CSB-EP301889TPR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Alanine--tRNA ligase (alaS), partial CSB-YP301890TNT
CSB-EP301890TNT
CSB-BP301890TNT
CSB-MP301890TNT
CSB-EP301890TNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hemin import ATP-binding protein HmuV (hmuV) CSB-YP301891YAQ
CSB-EP301891YAQ
CSB-BP301891YAQ
CSB-MP301891YAQ
CSB-EP301891YAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Proton extrusion protein PcxA (pcxA), partial CSB-YP301892SSQ1
CSB-EP301892SSQ1
CSB-BP301892SSQ1
CSB-MP301892SSQ1
CSB-EP301892SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative 1-phosphofructokinase (fruK) CSB-YP301893MLW
CSB-EP301893MLW
CSB-BP301893MLW
CSB-MP301893MLW
CSB-EP301893MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Ribose-phosphate pyrophosphokinase (prs) CSB-YP301894MLW
CSB-EP301894MLW
CSB-BP301894MLW
CSB-MP301894MLW
CSB-EP301894MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Spermidine/putrescine transport system permease protein PotB homolog (potB), partial CSB-YP301895MLW1
CSB-EP301895MLW1
CSB-BP301895MLW1
CSB-MP301895MLW1
CSB-EP301895MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_049 (MPN_049), partial CSB-YP301896MLW
CSB-EP301896MLW
CSB-BP301896MLW
CSB-MP301896MLW
CSB-EP301896MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Probable glycerol uptake facilitator protein (glpF), partial CSB-YP301897MLW1
CSB-EP301897MLW1
CSB-BP301897MLW1
CSB-MP301897MLW1
CSB-EP301897MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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