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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG029 homolog (MPN_032) CSB-YP301898MLW
CSB-EP301898MLW
CSB-BP301898MLW
CSB-MP301898MLW
CSB-EP301898MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Probable DNA-directed RNA polymerase subunit delta (rpoE) CSB-YP301899MLW
CSB-EP301899MLW
CSB-BP301899MLW
CSB-MP301899MLW
CSB-EP301899MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG012 homolog (MPN_016) CSB-YP301900MLW
CSB-EP301900MLW
CSB-BP301900MLW
CSB-MP301900MLW
CSB-EP301900MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_011 (MPN_011) CSB-YP301901MLW
CSB-EP301901MLW
CSB-BP301901MLW
CSB-MP301901MLW
CSB-EP301901MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized ABC transporter permease MG468 homolog (MPN_684), partial CSB-YP301902MLW
CSB-EP301902MLW
CSB-BP301902MLW
CSB-MP301902MLW
CSB-EP301902MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0134 protein MPN_675 (MPN_675) CSB-YP301903MLW
CSB-EP301903MLW
CSB-BP301903MLW
CSB-MP301903MLW
CSB-EP301903MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG452 homolog (MPN_666), partial CSB-YP301904MLW1
CSB-EP301904MLW1
CSB-BP301904MLW1
CSB-MP301904MLW1
CSB-EP301904MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 30S ribosomal protein S16 (rpsP) CSB-YP301905MLW
CSB-EP301905MLW
CSB-BP301905MLW
CSB-MP301905MLW
CSB-EP301905MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_654 (MPN_654) CSB-YP301906MLW
CSB-EP301906MLW
CSB-BP301906MLW
CSB-MP301906MLW
CSB-EP301906MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae PTS system mannitol-specific EIICB component (mtlA), partial CSB-YP301907MLW
CSB-EP301907MLW
CSB-BP301907MLW
CSB-MP301907MLW
CSB-EP301907MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MG440 homolog 3 (MPN_641) CSB-YP301908MLW
CSB-EP301908MLW
CSB-BP301908MLW
CSB-MP301908MLW
CSB-EP301908MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_634 (MPN_634) CSB-YP301909MLW
CSB-EP301909MLW
CSB-BP301909MLW
CSB-MP301909MLW
CSB-EP301909MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG427 homolog (MPN_625) CSB-YP301910MLW
CSB-EP301910MLW
CSB-BP301910MLW
CSB-MP301910MLW
CSB-EP301910MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae DNA polymerase III subunit gamma/tau (dnaX), partial CSB-YP301911MLW
CSB-EP301911MLW
CSB-BP301911MLW
CSB-MP301911MLW
CSB-EP301911MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Phosphate transport system permease protein pstA homolog (pstA), partial CSB-YP301912MLW
CSB-EP301912MLW
CSB-BP301912MLW
CSB-MP301912MLW
CSB-EP301912MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_584 (MPN_584) CSB-YP301913MLW
CSB-EP301913MLW
CSB-BP301913MLW
CSB-MP301913MLW
CSB-EP301913MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 10 kDa chaperonin (groS) CSB-YP301914MLW
CSB-EP301914MLW
CSB-BP301914MLW
CSB-MP301914MLW
CSB-EP301914MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Protein P200 (p200), partial CSB-YP301915MLW
CSB-BP301915MLW
CSB-MP301915MLW
CSB-EP301915MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Probable NH (3)-dependent NAD (+) synthetase CSB-YP301916MLW
CSB-EP301916MLW
CSB-BP301916MLW
CSB-MP301916MLW
CSB-EP301916MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG376 homolog (MPN_554) CSB-YP301917MLW
CSB-EP301917MLW
CSB-BP301917MLW
CSB-MP301917MLW
CSB-EP301917MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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