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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Tryptophan synthase alpha chain (trpA) CSB-YP302894SSQ
CSB-EP302894SSQ
CSB-BP302894SSQ
CSB-MP302894SSQ
CSB-EP302894SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cryptochrome DASH (cry) CSB-YP302895SSQ
CSB-EP302895SSQ
CSB-BP302895SSQ
CSB-MP302895SSQ
CSB-EP302895SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 3-dehydroquinate synthase (aroB) CSB-YP302896SXB
CSB-EP302896SXB
CSB-BP302896SXB
CSB-MP302896SXB
CSB-EP302896SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermotoga maritima DNA gyrase subunit B (gyrB), partial CSB-YP302897TNJ
CSB-EP302897TNJ
CSB-BP302897TNJ
CSB-MP302897TNJ
CSB-EP302897TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L34 (rpmH) CSB-YP302898MLW
CSB-EP302898MLW
CSB-BP302898MLW
CSB-MP302898MLW
CSB-EP302898MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 60 kDa chaperonin (groL), partial CSB-YP302899MLW
CSB-EP302899MLW
CSB-BP302899MLW
CSB-MP302899MLW
CSB-EP302899MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L31 (rpmE) CSB-YP302900MLW
CSB-EP302900MLW
CSB-BP302900MLW
CSB-MP302900MLW
CSB-EP302900MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L21 (rplU) CSB-YP302901MLW
CSB-EP302901MLW
CSB-BP302901MLW
CSB-MP302901MLW
CSB-EP302901MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans mRNA-capping enzyme subunit alpha (CGT1) CSB-YP302902CZF
CSB-EP302902CZF
CSB-BP302902CZF
CSB-MP302902CZF
CSB-EP302902CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Catalase B (catB), partial CSB-YP302903EKF
CSB-EP302903EKF
CSB-BP302903EKF
CSB-MP302903EKF
CSB-EP302903EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Mating-type pheromone BBP1 (3), partial CSB-YP302905SXR
CSB-EP302905SXR
CSB-BP302905SXR
CSB-MP302905SXR
CSB-EP302905SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit delta 2 isoform (par2), partial CSB-YP302906SXV
CSB-EP302906SXV
CSB-BP302906SXV
CSB-MP302906SXV
CSB-EP302906SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C1919.03c (SPCC1919.03c) CSB-YP302907SXV
CSB-EP302907SXV
CSB-BP302907SXV
CSB-MP302907SXV
CSB-EP302907SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized alpha-1,2-galactosyltransferase C637.06 (SPAC637.06), partial CSB-YP302908SXV
CSB-EP302908SXV
CSB-BP302908SXV
CSB-MP302908SXV
CSB-EP302908SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C21B10.08c (SPBC21B10.08c) CSB-YP302909SXV
CSB-EP302909SXV
CSB-BP302909SXV
CSB-MP302909SXV
CSB-EP302909SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Zinc finger protein rst2 (rst2), partial CSB-YP302910SXV
CSB-EP302910SXV
CSB-BP302910SXV
CSB-MP302910SXV
CSB-EP302910SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Division mal foutue 1 protein (mid1), partial CSB-YP302911SXV
CSB-EP302911SXV
CSB-BP302911SXV
CSB-MP302911SXV
CSB-EP302911SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Transcription factor atf21 (atf21) CSB-YP302912SXV
CSB-EP302912SXV
CSB-BP302912SXV
CSB-MP302912SXV
CSB-EP302912SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Sphingosine N-acyltransferase lag1 (lag1), partial CSB-YP302913SXV1
CSB-EP302913SXV1
CSB-BP302913SXV1
CSB-MP302913SXV1
CSB-EP302913SXV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alpha-L-arabinofuranosidase axhA (axhA) CSB-YP302914DOZ
CSB-EP302914DOZ
CSB-BP302914DOZ
CSB-MP302914DOZ
CSB-EP302914DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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