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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Thermotoga maritima RNA polymerase sigma factor rpoD (rpoD) CSB-YP302080TNJ
CSB-EP302080TNJ
CSB-BP302080TNJ
CSB-MP302080TNJ
CSB-EP302080TNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP302081MLW
CSB-EP302081MLW
CSB-BP302081MLW
CSB-MP302081MLW
CSB-EP302081MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Protein grpE (grpE) CSB-YP302082MLW
CSB-EP302082MLW
CSB-BP302082MLW
CSB-MP302082MLW
CSB-EP302082MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Carbamate kinase-like protein (MPN_307) CSB-YP302083MLW
CSB-EP302083MLW
CSB-BP302083MLW
CSB-MP302083MLW
CSB-EP302083MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Gamma-glutamylputrescine synthetase PuuA (puuA) CSB-YP302084ENV
CSB-EP302084ENV
CSB-BP302084ENV
CSB-MP302084ENV
CSB-EP302084ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable lysozyme from lambdoid prophage DLP12 (arrD) CSB-YP302085ENV
CSB-EP302085ENV
CSB-BP302085ENV
CSB-MP302085ENV
CSB-EP302085ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 26S protease regulatory subunit 6B homolog (tbpA) CSB-YP302086DOZ
CSB-EP302086DOZ
CSB-BP302086DOZ
CSB-MP302086DOZ
CSB-EP302086DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Elongation factor 1-beta (EFB1) CSB-YP302087CZE
CSB-EP302087CZE
CSB-BP302087CZE
CSB-MP302087CZE
CSB-EP302087CZE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coprinopsis cinerea Vesicular-fusion protein SEC17 (SEC17) CSB-YP302088DWQ
CSB-EP302088DWQ
CSB-BP302088DWQ
CSB-MP302088DWQ
CSB-EP302088DWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Cytokinesis protein sepA (sepA), partial CSB-YP302089EKF
CSB-EP302089EKF
CSB-BP302089EKF
CSB-MP302089EKF
CSB-EP302089EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Acriflavine sensitivity control protein acr-2 (acr-2), partial CSB-YP302090NHA
CSB-EP302090NHA
CSB-BP302090NHA
CSB-MP302090NHA
CSB-EP302090NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa White collar 2 protein (wc-2), partial CSB-YP302091NHA
CSB-EP302091NHA
CSB-BP302091NHA
CSB-MP302091NHA
CSB-EP302091NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Penicillium janthinellum Aspartic protease pepA (pepA) CSB-YP302092ETK
CSB-EP302092ETK
CSB-BP302092ETK
CSB-MP302092ETK
CSB-EP302092ETK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized proline-rich protein C70.10 (SPCC70.10) CSB-YP302093SXV
CSB-EP302093SXV
CSB-BP302093SXV
CSB-MP302093SXV
CSB-EP302093SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe ENTH domain-containing protein C794.11c (SPCC794.11c) CSB-YP302094SXV
CSB-EP302094SXV
CSB-BP302094SXV
CSB-MP302094SXV
CSB-EP302094SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe High-affinity glucose transporter ght5 (ght5), partial CSB-YP302095SXV
CSB-EP302095SXV
CSB-BP302095SXV
CSB-MP302095SXV
CSB-EP302095SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Probable S- (hydroxymethyl)glutathione dehydrogenase 1 CSB-YP302096SXV
CSB-EP302096SXV
CSB-BP302096SXV
CSB-MP302096SXV
CSB-EP302096SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Putative nucleosome assembly protein C2D10.11C (SPBC2D10.11c) CSB-YP302097SXV
CSB-EP302097SXV
CSB-BP302097SXV
CSB-MP302097SXV
CSB-EP302097SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein arginine N-methyltransferase skb1 (skb1), partial CSB-YP302098SXV
CSB-EP302098SXV
CSB-BP302098SXV
CSB-MP302098SXV
CSB-EP302098SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe WD repeat-containing protein slp1 (slp1) CSB-YP302099SXV
CSB-EP302099SXV
CSB-BP302099SXV
CSB-MP302099SXV
CSB-EP302099SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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