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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli N-acyltransferase YncA (yncA) CSB-YP301999ENV
CSB-EP301999ENV
CSB-BP301999ENV
CSB-MP301999ENV
CSB-EP301999ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized GST-like protein yncG (yncG) CSB-YP302000ENV
CSB-EP302000ENV
CSB-BP302000ENV
CSB-MP302000ENV
CSB-EP302000ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein bdm (bdm) CSB-YP302001ENV
CSB-EP302001ENV
CSB-BP302001ENV
CSB-MP302001ENV
CSB-EP302001ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ydet (ydeT) CSB-YP302002ENV
CSB-EP302002ENV
CSB-BP302002ENV
CSB-MP302002ENV
CSB-EP302002ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YneF (yneF), partial CSB-YP302003ENV1
CSB-EP302003ENV1
CSB-BP302003ENV1
CSB-MP302003ENV1
CSB-EP302003ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ynfN (ynfN) CSB-YP302004ENV
CSB-EP302004ENV
CSB-BP302004ENV
CSB-MP302004ENV
CSB-EP302004ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydfX (ydfX) CSB-YP302005ENV
CSB-EP302005ENV
CSB-BP302005ENV
CSB-MP302005ENV
CSB-EP302005ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Anaerobic dimethyl sulfoxide reductase chain ynfH (ynfH), partial CSB-YP302006ENV
CSB-EP302006ENV
CSB-BP302006ENV
CSB-MP302006ENV
CSB-EP302006ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydhJ (ydhJ), partial CSB-YP302007ENV
CSB-EP302007ENV
CSB-BP302007ENV
CSB-MP302007ENV
CSB-EP302007ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative electron transfer flavoprotein subunit ydiQ (ydiQ) CSB-YP302008ENV
CSB-EP302008ENV
CSB-BP302008ENV
CSB-MP302008ENV
CSB-EP302008ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Excinuclease cho (cho) CSB-YP302009ENV
CSB-EP302009ENV
CSB-BP302009ENV
CSB-MP302009ENV
CSB-EP302009ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli TVP38/TMEM64 family inner membrane protein ydjZ (ydjZ), partial CSB-YP302010ENV1
CSB-EP302010ENV1
CSB-BP302010ENV1
CSB-MP302010ENV1
CSB-EP302010ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yeaC (yeaC) CSB-YP302011ENV
CSB-EP302011ENV
CSB-BP302011ENV
CSB-MP302011ENV
CSB-EP302011ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator yeaM (yeaM) CSB-YP302012ENV
CSB-EP302012ENV
CSB-BP302012ENV
CSB-MP302012ENV
CSB-EP302012ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli D-malate dehydrogenase [decarboxylating] (dmlA) CSB-YP302013ENV
CSB-EP302013ENV
CSB-BP302013ENV
CSB-MP302013ENV
CSB-EP302013ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized transporter yebQ (yebQ), partial CSB-YP302014ENV
CSB-EP302014ENV
CSB-BP302014ENV
CSB-MP302014ENV
CSB-EP302014ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA (mo5U34)-methyltransferase CSB-YP302015ENV
CSB-EP302015ENV
CSB-BP302015ENV
CSB-MP302015ENV
CSB-EP302015ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yedK (yedK) CSB-YP302016ENV
CSB-EP302016ENV
CSB-BP302016ENV
CSB-MP302016ENV
CSB-EP302016ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein yedR (yedR), partial CSB-YP302017ENV1
CSB-EP302017ENV1
CSB-BP302017ENV1
CSB-MP302017ENV1
CSB-EP302017ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Metal-binding protein ZinT (zinT) CSB-YP302018ENV
CSB-EP302018ENV
CSB-BP302018ENV
CSB-MP302018ENV
CSB-EP302018ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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