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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Phycobilisome 32.1 kDa linker polypeptide, phycocyanin-associated, rod 2 (cpcC2) CSB-YP302632SSQ
CSB-EP302632SSQ
CSB-BP302632SSQ
CSB-MP302632SSQ
CSB-EP302632SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP302633SSQ
CSB-EP302633SSQ
CSB-BP302633SSQ
CSB-MP302633SSQ
CSB-EP302633SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Adenylosuccinate synthetase (purA) CSB-YP302634SSQ
CSB-EP302634SSQ
CSB-BP302634SSQ
CSB-MP302634SSQ
CSB-EP302634SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S11 (rpsK) CSB-YP302635SSQ
CSB-EP302635SSQ
CSB-BP302635SSQ
CSB-MP302635SSQ
CSB-EP302635SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S5 (rpsE) CSB-YP302636SSQ
CSB-EP302636SSQ
CSB-BP302636SSQ
CSB-MP302636SSQ
CSB-EP302636SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S17 (rpsQ) CSB-YP302637SSQ
CSB-EP302637SSQ
CSB-BP302637SSQ
CSB-MP302637SSQ
CSB-EP302637SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 50S ribosomal protein L3 (rplC) CSB-YP302638SSQ
CSB-EP302638SSQ
CSB-BP302638SSQ
CSB-MP302638SSQ
CSB-EP302638SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0145 protein sll118 (sll1118) CSB-YP302639SSQ
CSB-EP302639SSQ
CSB-BP302639SSQ
CSB-MP302639SSQ
CSB-EP302639SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein sll1735 (sll1735) CSB-YP302640SSQ
CSB-EP302640SSQ
CSB-BP302640SSQ
CSB-MP302640SSQ
CSB-EP302640SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA-binding protein HU (hup) CSB-YP302641SSQ
CSB-EP302641SSQ
CSB-BP302641SSQ
CSB-MP302641SSQ
CSB-EP302641SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cell division protein FtsZ (ftsZ) CSB-YP302643SSQ
CSB-EP302643SSQ
CSB-BP302643SSQ
CSB-MP302643SSQ
CSB-EP302643SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable glutaredoxin ssr2061 (ssr2061) CSB-YP302644SSQ
CSB-EP302644SSQ
CSB-BP302644SSQ
CSB-MP302644SSQ
CSB-EP302644SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 6,7-dimethyl-8-ribityllumazine synthase (ribH) CSB-YP302645SSQ
CSB-EP302645SSQ
CSB-BP302645SSQ
CSB-MP302645SSQ
CSB-EP302645SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Biotin synthase (bioB) CSB-YP302646SSQ
CSB-EP302646SSQ
CSB-BP302646SSQ
CSB-MP302646SSQ
CSB-EP302646SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP302647SSQ
CSB-EP302647SSQ
CSB-BP302647SSQ
CSB-MP302647SSQ
CSB-EP302647SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized WD repeat-containing protein slr1410 (slr1410) CSB-YP302648SSQ
CSB-EP302648SSQ
CSB-BP302648SSQ
CSB-MP302648SSQ
CSB-EP302648SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative quercetin 2,3-dioxygenase sll1773 (sll1773) CSB-YP302649SSQ
CSB-EP302649SSQ
CSB-BP302649SSQ
CSB-MP302649SSQ
CSB-EP302649SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. ATP-dependent Clp protease adapter protein ClpS (clpS) CSB-YP302650SSQ
CSB-EP302650SSQ
CSB-BP302650SSQ
CSB-MP302650SSQ
CSB-EP302650SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Tryptophan--tRNA ligase (trpS) CSB-YP302651SSQ
CSB-EP302651SSQ
CSB-BP302651SSQ
CSB-MP302651SSQ
CSB-EP302651SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UDP-N-acetylmuramoylalanine--D-glutamate ligase (murD) CSB-YP302652SSQ
CSB-EP302652SSQ
CSB-BP302652SSQ
CSB-MP302652SSQ
CSB-EP302652SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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