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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Mycobacterium tuberculosis Serine/threonine-protein kinase pknF (pknF), partial CSB-YP302592MVZ1
CSB-EP302592MVZ1
CSB-BP302592MVZ1
CSB-MP302592MVZ1
CSB-EP302592MVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrodipicolinate reductase (dapB) CSB-YP302593MVZ
CSB-EP302593MVZ
CSB-BP302593MVZ
CSB-MP302593MVZ
CSB-EP302593MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabinofuranosyltransferase AftA (aftA), partial CSB-YP302594MVZ
CSB-EP302594MVZ
CSB-BP302594MVZ
CSB-MP302594MVZ
CSB-EP302594MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Natronomonas pharaonis Succinate dehydrogenase hydrophobic membrane anchor subunit (sdhD), partial CSB-YP302595NAX
CSB-EP302595NAX
CSB-BP302595NAX
CSB-MP302595NAX
CSB-EP302595NAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa tRNA pseudouridine synthase B (truB) CSB-YP302596EZX
CSB-EP302596EZX
CSB-BP302596EZX
CSB-MP302596EZX
CSB-EP302596EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Ornithine utilization regulator (oruR) CSB-YP302597EZX
CSB-EP302597EZX
CSB-BP302597EZX
CSB-MP302597EZX
CSB-EP302597EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus Pyrolysin (pls), partial CSB-YP302598FHW
CSB-EP302598FHW
CSB-BP302598FHW
CSB-MP302598FHW
CSB-EP302598FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella haemolytica Protein CysZ homolog (cysZ), partial CSB-YP302599ESE
CSB-EP302599ESE
CSB-BP302599ESE
CSB-MP302599ESE
CSB-EP302599ESE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ispG) CSB-YP302601EZG
CSB-EP302601EZG
CSB-BP302601EZG
CSB-MP302601EZG
CSB-EP302601EZG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus ATP synthase epsilon chain (atpC) CSB-YP302602RLC
CSB-EP302602RLC
CSB-BP302602RLC
CSB-MP302602RLC
CSB-EP302602RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubrivivax gelatinosus Light-harvesting protein B-800/850 beta chain (pucB), partial CSB-YP302603RHU
CSB-EP302603RHU
CSB-BP302603RHU
CSB-MP302603RHU
CSB-EP302603RHU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Octopine transport system permease protein occM (occM), partial CSB-YP302604RKT1
CSB-EP302604RKT1
CSB-BP302604RKT1
CSB-MP302604RKT1
CSB-EP302604RKT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Nodulation protein G (nodG) CSB-YP302605RCY
CSB-EP302605RCY
CSB-BP302605RCY
CSB-MP302605RCY
CSB-EP302605RCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alcaligenes xylosoxydans xylosoxydans D-aminoacylase (dan) CSB-YP302606AGR
CSB-EP302606AGR
CSB-BP302606AGR
CSB-MP302606AGR
CSB-EP302606AGR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor 3-oxoacyl-[acyl-carrier-protein] synthase 3 protein 1 (fabH1) CSB-YP302607FOB
CSB-EP302607FOB
CSB-BP302607FOB
CSB-MP302607FOB
CSB-EP302607FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligopeptide transport ATP-binding protein OppF (oppF) CSB-YP302608FMR
CSB-EP302608FMR
CSB-BP302608FMR
CSB-MP302608FMR
CSB-EP302608FMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Allophycocyanin beta chain (apcB) CSB-YP302609FKP
CSB-EP302609FKP
CSB-BP302609FKP
CSB-MP302609FKP
CSB-EP302609FKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C-phycocyanin alpha chain (cpcA) CSB-YP302610FKP
CSB-EP302610FKP
CSB-BP302610FKP
CSB-MP302610FKP
CSB-EP302610FKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Prenyl transferase (preA) CSB-YP302611SSQ
CSB-EP302611SSQ
CSB-BP302611SSQ
CSB-MP302611SSQ
CSB-EP302611SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. UPF0182 protein sll1060 (sll1060), partial CSB-YP302612SSQ
CSB-EP302612SSQ
CSB-BP302612SSQ
CSB-MP302612SSQ
CSB-EP302612SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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