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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Serine/threonine-protein kinase B (spkB), partial CSB-YP302673SSQ
CSB-EP302673SSQ
CSB-BP302673SSQ
CSB-MP302673SSQ
CSB-EP302673SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA-directed RNA polymerase subunit omega (rpoZ) CSB-YP302674SSQ
CSB-EP302674SSQ
CSB-BP302674SSQ
CSB-MP302674SSQ
CSB-EP302674SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ribonuclease 3 1 (rnc1) CSB-YP302675SSQ
CSB-EP302675SSQ
CSB-BP302675SSQ
CSB-MP302675SSQ
CSB-EP302675SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 30S ribosomal protein S16 (rpsP) CSB-YP302676SSQ
CSB-EP302676SSQ
CSB-BP302676SSQ
CSB-MP302676SSQ
CSB-EP302676SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized transporter sll0355 (sll0355), partial CSB-YP302677SSQ
CSB-EP302677SSQ
CSB-BP302677SSQ
CSB-MP302677SSQ
CSB-EP302677SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable ATP-dependent Clp protease proteolytic subunit 3 (clpP3) CSB-YP302678SSQ
CSB-EP302678SSQ
CSB-BP302678SSQ
CSB-MP302678SSQ
CSB-EP302678SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI), partial CSB-YP302679SSQ
CSB-EP302679SSQ
CSB-BP302679SSQ
CSB-MP302679SSQ
CSB-EP302679SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glycogen synthase 1 (glgA1) CSB-YP302680SSQ
CSB-EP302680SSQ
CSB-BP302680SSQ
CSB-MP302680SSQ
CSB-EP302680SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Allophycocyanin subunit beta-18 (apcF) CSB-YP302681SSQ
CSB-EP302681SSQ
CSB-BP302681SSQ
CSB-MP302681SSQ
CSB-EP302681SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr1565 (slr1565) CSB-YP302682SSQ
CSB-EP302682SSQ
CSB-BP302682SSQ
CSB-MP302682SSQ
CSB-EP302682SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized transporter slr0753 (slr0753), partial CSB-YP302683SSQ
CSB-EP302683SSQ
CSB-BP302683SSQ
CSB-MP302683SSQ
CSB-EP302683SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative Holliday junction resolvase (sll1547) CSB-YP302684SSQ
CSB-EP302684SSQ
CSB-BP302684SSQ
CSB-MP302684SSQ
CSB-EP302684SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Bifunctional purine biosynthesis protein PurH (purH), partial CSB-YP302685SSQ
CSB-EP302685SSQ
CSB-BP302685SSQ
CSB-MP302685SSQ
CSB-EP302685SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharolobus shibatae Reverse gyrase (rgy), partial CSB-YP302686FPJ
CSB-EP302686FPJ
CSB-BP302686FPJ
CSB-MP302686FPJ
CSB-EP302686FPJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. Photosystem II 12 kDa extrinsic protein (psbU) CSB-YP302687FQA
CSB-EP302687FQA
CSB-BP302687FQA
CSB-MP302687FQA
CSB-EP302687FQA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem II reaction center protein T (psbT), partial CSB-YP302688SSQ
CSB-EP302688SSQ
CSB-BP302688SSQ
CSB-MP302688SSQ
CSB-EP302688SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 2-methylcitrate dehydratase (prpD) CSB-YP302689SXB
CSB-EP302689SXB
CSB-BP302689SXB
CSB-MP302689SXB
CSB-EP302689SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Secretion system apparatus protein ssaL (ssaL) CSB-YP302690SXB
CSB-EP302690SXB
CSB-BP302690SXB
CSB-MP302690SXB
CSB-EP302690SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium DNA polymerase III subunit tau (dnaX), partial CSB-YP302691SXB
CSB-EP302691SXB
CSB-BP302691SXB
CSB-MP302691SXB
CSB-EP302691SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Secretion system apparatus protein SsaS (ssaS), partial CSB-YP302692SXB1
CSB-EP302692SXB1
CSB-BP302692SXB1
CSB-MP302692SXB1
CSB-EP302692SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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