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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Pyrimidine monooxygenase RutA (rutA) CSB-YP302793ENV
CSB-EP302793ENV
CSB-BP302793ENV
CSB-MP302793ENV
CSB-EP302793ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ycdU (ycdU), partial CSB-YP302794ENV
CSB-EP302794ENV
CSB-BP302794ENV
CSB-MP302794ENV
CSB-EP302794ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YmdC (ymdC) CSB-YP302795ENV
CSB-EP302795ENV
CSB-BP302795ENV
CSB-MP302795ENV
CSB-EP302795ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Flagellar basal-body rod protein flgF (flgF) CSB-YP302796ENV
CSB-EP302796ENV
CSB-BP302796ENV
CSB-MP302796ENV
CSB-EP302796ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein YcfT (ycfT), partial CSB-YP302797ENV
CSB-EP302797ENV
CSB-BP302797ENV
CSB-MP302797ENV
CSB-EP302797ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribosomal large subunit pseudouridine synthase E (rluE) CSB-YP302798ENV
CSB-EP302798ENV
CSB-BP302798ENV
CSB-MP302798ENV
CSB-EP302798ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ymfJ (ymfJ) CSB-YP302799ENV
CSB-EP302799ENV
CSB-BP302799ENV
CSB-MP302799ENV
CSB-EP302799ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative protein BeeE from lambdoid prophage e14 region (beeE) CSB-YP302800ENV
CSB-EP302800ENV
CSB-BP302800ENV
CSB-MP302800ENV
CSB-EP302800ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable two-component-system connector protein YcgZ (ycgZ) CSB-YP302801ENV
CSB-EP302801ENV
CSB-BP302801ENV
CSB-MP302801ENV
CSB-EP302801ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inhibitor of g-type lysozyme (pliG) CSB-YP302802ENV
CSB-EP302802ENV
CSB-BP302802ENV
CSB-MP302802ENV
CSB-EP302802ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YcgV (ycgV), partial CSB-YP302803ENV
CSB-EP302803ENV
CSB-BP302803ENV
CSB-MP302803ENV
CSB-EP302803ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative sucrose phosphorylase (ycjM) CSB-YP302804ENV
CSB-EP302804ENV
CSB-BP302804ENV
CSB-MP302804ENV
CSB-EP302804ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ycjY (ycjY) CSB-YP302805ENV
CSB-EP302805ENV
CSB-BP302805ENV
CSB-MP302805ENV
CSB-EP302805ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Rac prophage repressor (racR) CSB-YP302806ENV
CSB-EP302806ENV
CSB-BP302806ENV
CSB-MP302806ENV
CSB-EP302806ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transposase insH for insertion sequence element IS5Y (insH5) CSB-YP302807ENV
CSB-EP302807ENV
CSB-BP302807ENV
CSB-MP302807ENV
CSB-EP302807ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative 1,2-phenylacetyl-CoA epoxidase, subunit D (paaD) CSB-YP302808ENV
CSB-EP302808ENV
CSB-BP302808ENV
CSB-MP302808ENV
CSB-EP302808ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Transcriptional repressor PaaX (paaX) CSB-YP302809ENV
CSB-EP302809ENV
CSB-BP302809ENV
CSB-MP302809ENV
CSB-EP302809ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydcJ (ydcJ) CSB-YP302810ENV
CSB-EP302810ENV
CSB-BP302810ENV
CSB-MP302810ENV
CSB-EP302810ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein ydcZ (ydcZ), partial CSB-YP302811ENV1
CSB-EP302811ENV1
CSB-BP302811ENV1
CSB-MP302811ENV1
CSB-EP302811ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YncE (yncE) CSB-YP302812ENV
CSB-BP302812ENV
CSB-MP302812ENV
CSB-EP302812ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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