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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant 60 kDa chaperonin (groL), partial CSB-YP303202SMT
CSB-EP303202SMT
CSB-BP303202SMT
CSB-MP303202SMT
CSB-EP303202SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Formyl-coenzyme A transferase (frc) CSB-YP303203ENV
CSB-EP303203ENV
CSB-BP303203ENV
CSB-MP303203ENV
CSB-EP303203ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glutamate decarboxylase beta (gadB) CSB-YP303204EOD
CSB-EP303204EOD
CSB-BP303204EOD
CSB-MP303204EOD
CSB-EP303204EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Carbon storage regulator (csrA) CSB-YP303205SXB
CSB-EP303205SXB
CSB-BP303205SXB
CSB-MP303205SXB
CSB-EP303205SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DnaA-homolog protein hda (hda) CSB-YP303207EGX
CSB-EP303207EGX
CSB-BP303207EGX
CSB-MP303207EGX
CSB-EP303207EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tityus serrulatus Potassium channel toxin TsTXK-beta CSB-YP303208TON
CSB-EP303208TON
CSB-BP303208TON
CSB-MP303208TON
CSB-EP303208TON-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anemonia erythraea Toxin AETX-3 CSB-YP303209AKD
CSB-EP303209AKD
CSB-BP303209AKD
CSB-MP303209AKD
CSB-EP303209AKD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP-dependent dethiobiotin synthetase BioD (bioD) CSB-YP303210YAH
CSB-EP303210YAH
CSB-BP303210YAH
CSB-MP303210YAH
CSB-EP303210YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Low calcium response locus protein S (lcrS) CSB-YP303211YAH
CSB-EP303211YAH
CSB-BP303211YAH
CSB-MP303211YAH
CSB-EP303211YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protein tyeA (tyeA) CSB-YP303212YAH
CSB-EP303212YAH
CSB-BP303212YAH
CSB-MP303212YAH
CSB-EP303212YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein K (yscK) CSB-YP303213YAH
CSB-EP303213YAH
CSB-BP303213YAH
CSB-MP303213YAH
CSB-EP303213YAH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yop proteins translocation protein R (yscR), partial CSB-YP303214YAH1
CSB-EP303214YAH1
CSB-BP303214YAH1
CSB-MP303214YAH1
CSB-EP303214YAH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Urease subunit beta (ureB) CSB-YP303215YAS
CSB-EP303215YAS
CSB-BP303215YAS
CSB-MP303215YAS
CSB-EP303215YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Urease subunit beta (ureB), partial CSB-YP303216HCQ
CSB-EP303216HCQ
CSB-BP303216HCQ
CSB-MP303216HCQ
CSB-EP303216HCQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis POU domain, class 3, transcription factor 2-B (pou3f2-b) CSB-YP303217XBE
CSB-EP303217XBE
CSB-BP303217XBE
CSB-MP303217XBE
CSB-EP303217XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Transcription factor 7-like 1-A (tcf7l1-a), partial CSB-YP303218XBE
CSB-EP303218XBE
CSB-BP303218XBE
CSB-MP303218XBE
CSB-EP303218XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trimeresurus gramineus Phospholipase A2 isozyme 7 CSB-YP303219TQK
CSB-EP303219TQK
CSB-BP303219TQK
CSB-MP303219TQK
CSB-EP303219TQK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Disintegrin and metalloproteinase domain-containing protein 1 (Adam1), partial CSB-YP303220RA
CSB-EP303220RA
CSB-BP303220RA
CSB-MP303220RA
CSB-EP303220RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aggregatibacter actinomycetemcomitans 3-oxoacyl-[acyl-carrier-protein] reductase FabG (fabG) CSB-YP303221AYK
CSB-EP303221AYK
CSB-BP303221AYK
CSB-MP303221AYK
CSB-EP303221AYK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Protein dsrD (dsrD) CSB-YP303222DOC
CSB-EP303222DOC
CSB-BP303222DOC
CSB-MP303222DOC
CSB-EP303222DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€(xiàn)留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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