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重組蛋白

產品名稱 貨號 規(guī)格
Recombinant Delta (14)-sterol reductase, partial CSB-YP304417APE
CSB-EP304417APE
CSB-BP304417APE
CSB-MP304417APE
CSB-EP304417APE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Proline--tRNA ligase, cytoplasmic (PRS), partial CSB-YP304418CZF
CSB-EP304418CZF
CSB-BP304418CZF
CSB-MP304418CZF
CSB-EP304418CZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Emericella nidulans Acid trehalase (treA), partial CSB-YP304419EKF
CSB-EP304419EKF
CSB-BP304419EKF
CSB-MP304419EKF
CSB-EP304419EKF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein SOM1, mitochondrial (SOM1), partial CSB-YP304420KBK
CSB-EP304420KBK
CSB-BP304420KBK
CSB-MP304420KBK
CSB-EP304420KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa V-type proton ATPase subunit G (vma-10) CSB-YP304421NHA
CSB-EP304421NHA
CSB-BP304421NHA
CSB-MP304421NHA
CSB-EP304421NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pichia angusta Peroxisomal membrane protein PER10 (PEX14) CSB-YP304422EVQ
CSB-EP304422EVQ
CSB-BP304422EVQ
CSB-MP304422EVQ
CSB-EP304422EVQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizophyllum commune Mating-type pheromone BBP1 (2), partial CSB-YP304423SXR
CSB-EP304423SXR
CSB-BP304423SXR
CSB-MP304423SXR
CSB-EP304423SXR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cytochrome b-c1 complex subunit 2, mitochondrial (qcr2) CSB-YP304424SXV
CSB-EP304424SXV
CSB-BP304424SXV
CSB-MP304424SXV
CSB-EP304424SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe NADP-specific glutamate dehydrogenase (gdh1) CSB-YP304425SXV
CSB-EP304425SXV
CSB-BP304425SXV
CSB-MP304425SXV
CSB-EP304425SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Pre-mRNA-splicing factor srp2 (srp2) CSB-YP304426SXV
CSB-EP304426SXV
CSB-BP304426SXV
CSB-MP304426SXV
CSB-EP304426SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Iron-sulfur assembly protein 1 (isa1) CSB-YP304427SXV
CSB-EP304427SXV
CSB-BP304427SXV
CSB-MP304427SXV
CSB-EP304427SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Uncharacterized protein C23H3.15c (SPAC23H3.15c, SPAC25H1.01c) CSB-YP304428SXV
CSB-EP304428SXV
CSB-BP304428SXV
CSB-MP304428SXV
CSB-EP304428SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Cofilin (cof1) CSB-YP304429SXV
CSB-EP304429SXV
CSB-BP304429SXV
CSB-MP304429SXV
CSB-EP304429SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces pastorianus Meiotic recombination protein REC114 (REC114) CSB-YP304430SAJ
CSB-EP304430SAJ
CSB-BP304430SAJ
CSB-MP304430SAJ
CSB-EP304430SAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Mating factor M secretion protein mam1 (mam1), partial CSB-YP304431SXV
CSB-EP304431SXV
CSB-BP304431SXV
CSB-MP304431SXV
CSB-EP304431SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Protein transport protein SEC61 subunit alpha (SEC61), partial CSB-YP304432YAP
CSB-EP304432YAP
CSB-BP304432YAP
CSB-MP304432YAP
CSB-EP304432YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 40S ribosomal protein S25-A (rps25a) CSB-YP304433SXV
CSB-EP304433SXV
CSB-BP304433SXV
CSB-MP304433SXV
CSB-EP304433SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fumigata Major allergen Asp f 2 (AFUA_4G09580) CSB-YP304434NGS
CSB-BP304434NGS
CSB-MP304434NGS
CSB-EP304434NGS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (ARO4) CSB-YP304435CZD
CSB-EP304435CZD
CSB-BP304435CZD
CSB-MP304435CZD
CSB-EP304435CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Protein translation factor sui1 (sui1) CSB-YP304436SXV
CSB-EP304436SXV
CSB-BP304436SXV
CSB-MP304436SXV
CSB-EP304436SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質形式,可以通過多種方式產生大量蛋白質、修改基因序列和制造有用的商業(yè)產品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據他們開發(fā)重組蛋白的經驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產,在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質合成機制來產生由該基因編碼的蛋白質。表達載體可使外源基因在宿主細胞中高效表達,經過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質?

根據重組蛋白的不同應用,對其生物活性有不同的要求。但是我們如何確保蛋白質的產生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質有任何疑問,涉及到蛋白質的價格、交貨、質量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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