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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Escherichia coli N-hydroxyarylamine O-acetyltransferase (nhoA) CSB-YP302874ENV
CSB-EP302874ENV
CSB-BP302874ENV
CSB-MP302874ENV
CSB-EP302874ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Succinylornithine transaminase (astC) CSB-YP302875ENV
CSB-EP302875ENV
CSB-BP302875ENV
CSB-MP302875ENV
CSB-EP302875ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative HTH-type transcriptional regulator ykgA (ykgA) CSB-YP302876ENV
CSB-EP302876ENV
CSB-BP302876ENV
CSB-MP302876ENV
CSB-EP302876ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UPF0214 protein yfeW (yfeW) CSB-YP302877ENV
CSB-EP302877ENV
CSB-BP302877ENV
CSB-MP302877ENV
CSB-EP302877ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ybcM (ybcM) CSB-YP302878ENV
CSB-EP302878ENV
CSB-BP302878ENV
CSB-MP302878ENV
CSB-EP302878ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autoinducer 2 import system permease protein lsrC (lsrC), partial CSB-YP302879ENV
CSB-EP302879ENV
CSB-BP302879ENV
CSB-MP302879ENV
CSB-EP302879ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (arnB) CSB-YP302880ENV
CSB-EP302880ENV
CSB-BP302880ENV
CSB-MP302880ENV
CSB-EP302880ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative tail fiber assembly protein homolog from lambdoid prophage DLP12 (tfaD) CSB-YP302881ENV
CSB-EP302881ENV
CSB-BP302881ENV
CSB-MP302881ENV
CSB-EP302881ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli tRNA sulfurtransferase (thiI) CSB-YP302882ENV
CSB-EP302882ENV
CSB-BP302882ENV
CSB-MP302882ENV
CSB-EP302882ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Oligopeptide transport ATP-binding protein OppF (oppF) CSB-YP302883ENV
CSB-EP302883ENV
CSB-BP302883ENV
CSB-MP302883ENV
CSB-EP302883ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ylbH (ylbH) CSB-YP302884ENV
CSB-EP302884ENV
CSB-BP302884ENV
CSB-MP302884ENV
CSB-EP302884ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein ybfO (ybfO) CSB-YP302885ENV
CSB-EP302885ENV
CSB-BP302885ENV
CSB-MP302885ENV
CSB-EP302885ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rubrivivax gelatinosus Light-harvesting protein B-800/850 alpha chain (pucA), partial CSB-YP302886RHU1
CSB-EP302886RHU1
CSB-BP302886RHU1
CSB-MP302886RHU1
CSB-EP302886RHU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Purine nucleoside phosphorylase 1 (punA) CSB-YP302887GFM
CSB-EP302887GFM
CSB-BP302887GFM
CSB-MP302887GFM
CSB-EP302887GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase-4 component C (hyfC), partial CSB-YP302888ENV
CSB-EP302888ENV
CSB-BP302888ENV
CSB-MP302888ENV
CSB-EP302888ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitrogenase molybdenum-iron protein alpha chain (nifD) CSB-YP302889HWA
CSB-EP302889HWA
CSB-BP302889HWA
CSB-MP302889HWA
CSB-EP302889HWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Aspartate carbamoyltransferase (pyrB) CSB-YP302890LMS
CSB-EP302890LMS
CSB-BP302890LMS
CSB-MP302890LMS
CSB-EP302890LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Glycine--tRNA ligase beta subunit (glyS) CSB-YP302891MIA
CSB-EP302891MIA
CSB-BP302891MIA
CSB-MP302891MIA
CSB-EP302891MIA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Putative bacterioferritin B (bfrB) CSB-YP302892NFZ
CSB-EP302892NFZ
CSB-BP302892NFZ
CSB-MP302892NFZ
CSB-EP302892NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable nicotinate-nucleotide pyrophosphorylase [carboxylating] (nadC) CSB-YP302893RMA
CSB-EP302893RMA
CSB-BP302893RMA
CSB-MP302893RMA
CSB-EP302893RMA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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