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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Schizosaccharomyces pombe Protein transport protein sec61 subunit alpha (sec61), partial CSB-YP302916SXV
CSB-EP302916SXV
CSB-BP302916SXV
CSB-MP302916SXV
CSB-EP302916SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Nitric oxide synthase, inducible (NOS2) CSB-YP302917PI
CSB-EP302917PI
CSB-BP302917PI
CSB-MP302917PI
CSB-EP302917PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Cathelicidin-2 (CATHL2) CSB-YP302918SH
CSB-EP302918SH
CSB-BP302918SH
CSB-MP302918SH
CSB-EP302918SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Metallothionein-1C (MT1C) CSB-YP302919PI
CSB-EP302919PI
CSB-BP302919PI
CSB-MP302919PI
CSB-EP302919PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3), partial CSB-YP302920HU
CSB-EP302920HU
CSB-BP302920HU
CSB-MP302920HU
CSB-EP302920HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Enterobacteria phage Mu Protein gp41 (41) CSB-YP302921EDA
CSB-EP302921EDA
CSB-BP302921EDA
CSB-MP302921EDA
CSB-EP302921EDA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix Vascular endothelial growth factor receptor 3 (FLT4), partial CSB-YP302922CRA
CSB-EP302922CRA
CSB-BP302922CRA
CSB-MP302922CRA
CSB-EP302922CRA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio kerri Tiggy-winkle hedgehog protein (twhh) CSB-YP302923DAI
CSB-EP302923DAI
CSB-BP302923DAI
CSB-MP302923DAI
CSB-EP302923DAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken T-box-containing protein TBXT (TBXT) CSB-YP302924CH
CSB-EP302924CH
CSB-BP302924CH
CSB-MP302924CH
CSB-EP302924CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryzias latipes Actin, cytoplasmic 1 (actb) CSB-YP302925OFH
CSB-EP302925OFH
CSB-BP302925OFH
CSB-MP302925OFH
CSB-EP302925OFH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Carboxy-terminal kinesin 2, partial CSB-YP302926XBE
CSB-EP302926XBE
CSB-BP302926XBE
CSB-MP302926XBE
CSB-EP302926XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sepia officinalis Spermatid-specific protein T2 CSB-YP302927SYJ
CSB-EP302927SYJ
CSB-BP302927SYJ
CSB-MP302927SYJ
CSB-EP302927SYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Azurocidin CSB-YP302928PI
CSB-EP302928PI
CSB-BP302928PI
CSB-MP302928PI
CSB-EP302928PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Parvalbumin, muscle CSB-YP302929CH
CSB-EP302929CH
CSB-BP302929CH
CSB-MP302929CH
CSB-EP302929CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zophobas atratus Coleoptericin CSB-YP302930ZAN
CSB-EP302930ZAN
CSB-BP302930ZAN
CSB-MP302930ZAN
CSB-EP302930ZAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Locusta migratoria Ovary maturating parsin CSB-YP302931LHY
CSB-EP302931LHY
CSB-BP302931LHY
CSB-MP302931LHY
CSB-EP302931LHY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Daucus carota Beta-fructofuranosidase, soluble isoenzyme I (INV*DC4), partial CSB-YP302932DIR
CSB-EP302932DIR
CSB-BP302932DIR
CSB-MP302932DIR
CSB-EP302932DIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Physcomitrella patens subsp. patens Myb-related protein Pp2 (PP2) CSB-YP302933EVD
CSB-EP302933EVD
CSB-BP302933EVD
CSB-MP302933EVD
CSB-EP302933EVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfolobus solfataricus Thermostable carboxypeptidase 1 (cpsA1) CSB-YP302934FPM
CSB-EP302934FPM
CSB-BP302934FPM
CSB-MP302934FPM
CSB-EP302934FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Halorhodospira halophila Light-harvesting protein B800/850/890 alpha-1 chain, partial CSB-YP302935HSG
CSB-EP302935HSG
CSB-BP302935HSG
CSB-MP302935HSG
CSB-EP302935HSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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