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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Synechocystis sp. Putative zinc metalloprotease slr1821 (slr1821), partial CSB-YP303324SSQ1
CSB-EP303324SSQ1
CSB-BP303324SSQ1
CSB-MP303324SSQ1
CSB-EP303324SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein sll1147 (sll1147), partial CSB-YP303326SSQ1
CSB-EP303326SSQ1
CSB-BP303326SSQ1
CSB-MP303326SSQ1
CSB-EP303326SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Aspartate--tRNA ligase (aspS), partial CSB-YP303327SSQ
CSB-EP303327SSQ
CSB-BP303327SSQ
CSB-MP303327SSQ
CSB-EP303327SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative sensor protein KdpD (kdpD) CSB-YP303328SSQ
CSB-EP303328SSQ
CSB-BP303328SSQ
CSB-MP303328SSQ
CSB-EP303328SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phycobilisome degradation protein nblA homolog 2 (ssl0453) CSB-YP303329SSQ
CSB-EP303329SSQ
CSB-BP303329SSQ
CSB-MP303329SSQ
CSB-EP303329SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Catalase-peroxidase (katG), partial CSB-YP303330SSQ
CSB-EP303330SSQ
CSB-BP303330SSQ
CSB-MP303330SSQ
CSB-EP303330SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Protein thf1 (thf1) CSB-YP303331SSQ
CSB-EP303331SSQ
CSB-BP303331SSQ
CSB-MP303331SSQ
CSB-EP303331SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Type III pantothenate kinase (coaX) CSB-YP303333SSQ
CSB-EP303333SSQ
CSB-BP303333SSQ
CSB-MP303333SSQ
CSB-EP303333SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Orange carotenoid-binding protein (slr1963) CSB-YP303334SSQ
CSB-EP303334SSQ
CSB-BP303334SSQ
CSB-MP303334SSQ
CSB-EP303334SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ycf55-like protein (sll1879), partial CSB-YP303335SSQ
CSB-EP303335SSQ
CSB-BP303335SSQ
CSB-MP303335SSQ
CSB-EP303335SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA primase (dnaG), partial CSB-YP303336SSQ
CSB-EP303336SSQ
CSB-BP303336SSQ
CSB-MP303336SSQ
CSB-EP303336SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA-directed RNA polymerase subunit gamma (rpoC1), partial CSB-YP303337SSQ
CSB-EP303337SSQ
CSB-BP303337SSQ
CSB-MP303337SSQ
CSB-EP303337SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP303338SSQ
CSB-EP303338SSQ
CSB-BP303338SSQ
CSB-MP303338SSQ
CSB-EP303338SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Putative carboxypeptidase slr1534 (slr1534) CSB-YP303339SSQ
CSB-EP303339SSQ
CSB-BP303339SSQ
CSB-MP303339SSQ
CSB-EP303339SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ribonucleoside-diphosphate reductase subunit alpha (nrdA), partial CSB-YP303340SSQ
CSB-EP303340SSQ
CSB-BP303340SSQ
CSB-MP303340SSQ
CSB-EP303340SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glycerol kinase (glpK) CSB-YP303341SSQ
CSB-EP303341SSQ
CSB-BP303341SSQ
CSB-MP303341SSQ
CSB-EP303341SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Isopentenyl-diphosphate delta-isomerase (fni) CSB-YP303342SSQ
CSB-EP303342SSQ
CSB-BP303342SSQ
CSB-MP303342SSQ
CSB-EP303342SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cobalt-precorrin-8X methylmutase (cbiC) CSB-YP303343SSQ
CSB-EP303343SSQ
CSB-BP303343SSQ
CSB-MP303343SSQ
CSB-EP303343SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Cysteine--tRNA ligase (cysS) CSB-YP303344SSQ
CSB-EP303344SSQ
CSB-BP303344SSQ
CSB-MP303344SSQ
CSB-EP303344SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable dihydroneopterin aldolase (folB) CSB-YP303345SSQ
CSB-EP303345SSQ
CSB-BP303345SSQ
CSB-MP303345SSQ
CSB-EP303345SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細(xì)胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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