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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Methanothermobacter thermautotrophicus Glycerol-1-phosphate dehydrogenase [NAD (P)+] CSB-YP304944MSR
CSB-EP304944MSR
CSB-BP304944MSR
CSB-MP304944MSR
CSB-EP304944MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Probable arabinosyltransferase B (embB), partial CSB-YP304945MVZ
CSB-EP304945MVZ
CSB-BP304945MVZ
CSB-MP304945MVZ
CSB-EP304945MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase (lst) CSB-YP304946NFZ
CSB-EP304946NFZ
CSB-BP304946NFZ
CSB-MP304946NFZ
CSB-EP304946NFZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Putative imidazole glycerol phosphate synthase subunit hisF2 (hisF2) CSB-YP304947EZX
CSB-EP304947EZX
CSB-BP304947EZX
CSB-MP304947EZX
CSB-EP304947EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Dihydroorotase (pyrC) CSB-YP304948EZX
CSB-EP304948EZX
CSB-BP304948EZX
CSB-MP304948EZX
CSB-EP304948EZX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis Lys-gingipain 381 (kgp), partial CSB-YP304949PQP
CSB-BP304949PQP
CSB-MP304949PQP
CSB-EP304949PQP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodococcus erythropolis Pup--protein ligase 2 (pafA2) CSB-YP304950RLJ
CSB-EP304950RLJ
CSB-BP304950RLJ
CSB-MP304950RLJ
CSB-EP304950RLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Opine oxidase subunit B (ooxB) CSB-YP304951RKT
CSB-EP304951RKT
CSB-BP304951RKT
CSB-MP304951RKT
CSB-EP304951RKT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium sp. Sulfate adenylyltransferase subunit 2 (nodP) CSB-YP304952RCY
CSB-EP304952RCY
CSB-BP304952RCY
CSB-MP304952RCY
CSB-EP304952RCY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Iron-sulfur cluster repair protein ScdA (scdA) CSB-YP304953FLF
CSB-EP304953FLF
CSB-BP304953FLF
CSB-MP304953FLF
CSB-EP304953FLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant C-phycocyanin beta chain (cpcB) CSB-YP304954FKP
CSB-EP304954FKP
CSB-BP304954FKP
CSB-MP304954FKP
CSB-EP304954FKP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized RNA pseudouridine synthase slr0612 (slr0612) CSB-YP304955SSQ
CSB-EP304955SSQ
CSB-BP304955SSQ
CSB-MP304955SSQ
CSB-EP304955SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Peptide methionine sulfoxide reductase MsrA 1 (msrA1) CSB-YP304956SSQ
CSB-EP304956SSQ
CSB-BP304956SSQ
CSB-MP304956SSQ
CSB-EP304956SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Phosphoribosylformylglycinamidine synthase 2 (purL), partial CSB-YP304957SSQ
CSB-EP304957SSQ
CSB-BP304957SSQ
CSB-MP304957SSQ
CSB-EP304957SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Probable signal peptidase I-1 (lepB1), partial CSB-YP304958SSQ
CSB-EP304958SSQ
CSB-BP304958SSQ
CSB-MP304958SSQ
CSB-EP304958SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ribosome maturation factor RimP (rimP) CSB-YP304959SSQ
CSB-EP304959SSQ
CSB-BP304959SSQ
CSB-MP304959SSQ
CSB-EP304959SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Photosystem I assembly protein Ycf4 (ycf4), partial CSB-YP304960SSQ1
CSB-EP304960SSQ1
CSB-BP304960SSQ1
CSB-MP304960SSQ1
CSB-EP304960SSQ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Uncharacterized protein slr1417 (slr1417) CSB-YP304961SSQ
CSB-EP304961SSQ
CSB-BP304961SSQ
CSB-MP304961SSQ
CSB-EP304961SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Carbon dioxide concentrating mechanism protein CcmL (ccmL) CSB-YP304962SSQ
CSB-EP304962SSQ
CSB-BP304962SSQ
CSB-MP304962SSQ
CSB-EP304962SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Ycf54-like protein (slr1780) CSB-YP304963SSQ
CSB-EP304963SSQ
CSB-BP304963SSQ
CSB-MP304963SSQ
CSB-EP304963SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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