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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant P4 prophage-derived uncharacterized protein t2654 (t2654) CSB-YP304739SWW
CSB-EP304739SWW
CSB-BP304739SWW
CSB-MP304739SWW
CSB-EP304739SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas phage phiLf Tail virion protein G7P (VII), partial CSB-YP304740XBD
CSB-EP304740XBD
CSB-BP304740XBD
CSB-MP304740XBD
CSB-EP304740XBD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 30S ribosomal protein S21 (rpsU) CSB-YP304741ENV
CSB-EP304741ENV
CSB-BP304741ENV
CSB-MP304741ENV
CSB-EP304741ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli ATP synthase subunit c (atpE), partial CSB-YP304742ENV1
CSB-EP304742ENV1
CSB-BP304742ENV1
CSB-MP304742ENV1
CSB-EP304742ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit c (atpE), partial CSB-YP304743SZB1
CSB-EP304743SZB1
CSB-BP304743SZB1
CSB-MP304743SZB1
CSB-EP304743SZB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Virion membrane protein A21 (MVA131L, ACAM3000_MVA_131), partial CSB-YP304744VEE
CSB-EP304744VEE
CSB-BP304744VEE
CSB-MP304744VEE
CSB-EP304744VEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Vaccinia virus Core protein A15 (VACWR135) CSB-YP304745VAI
CSB-EP304745VAI
CSB-BP304745VAI
CSB-MP304745VAI
CSB-EP304745VAI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesobuthus martensii Beta-insect depressant toxin BmKIT2 CSB-YP304746MRE
CSB-EP304746MRE
CSB-BP304746MRE
CSB-MP304746MRE
CSB-EP304746MRE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Bacillolysin (nprE) CSB-YP304747BRJ
CSB-EP304747BRJ
CSB-BP304747BRJ
CSB-MP304747BRJ
CSB-EP304747BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus Protein V118 (V118) CSB-YP304748AEE
CSB-EP304748AEE
CSB-BP304748AEE
CSB-MP304748AEE
CSB-EP304748AEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paeonia cambessedesii Maturase K (matK) CSB-YP304749PAJ
CSB-EP304749PAJ
CSB-BP304749PAJ
CSB-MP304749PAJ
CSB-EP304749PAJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant African swine fever virus IAP-like protein p27 (p27) CSB-YP304750AEH
CSB-EP304750AEH
CSB-BP304750AEH
CSB-MP304750AEH
CSB-EP304750AEH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor P (efp) CSB-YP304751SMT
CSB-EP304751SMT
CSB-BP304751SMT
CSB-MP304751SMT
CSB-EP304751SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Phospho-N-acetylmuramoyl-pentapeptide-transferase (mraY), partial CSB-YP304752SKY
CSB-EP304752SKY
CSB-BP304752SKY
CSB-MP304752SKY
CSB-EP304752SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus 3-oxoacyl-[acyl-carrier-protein] synthase 3 (fabH) CSB-YP304753SKX
CSB-EP304753SKX
CSB-BP304753SKX
CSB-MP304753SKX
CSB-EP304753SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspartyl/glutamyl-tRNA (Asn/Gln) amidotransferase subunit C CSB-YP304754FKZ
CSB-EP304754FKZ
CSB-BP304754FKZ
CSB-MP304754FKZ
CSB-EP304754FKZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Holliday junction resolvase recU (recU) CSB-YP304755SUL
CSB-EP304755SUL
CSB-BP304755SUL
CSB-MP304755SUL
CSB-EP304755SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Black beetle virus Protein B1 (B1) CSB-YP304756BHD
CSB-EP304756BHD
CSB-BP304756BHD
CSB-MP304756BHD
CSB-EP304756BHD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flock house virus Protein B2 (B2) CSB-YP304757FCA
CSB-EP304757FCA
CSB-BP304757FCA
CSB-MP304757FCA
CSB-EP304757FCA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Protein RecA (recA) CSB-YP304758SKX
CSB-EP304758SKX
CSB-BP304758SKX
CSB-MP304758SKX
CSB-EP304758SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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