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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Phenylacetate-coenzyme A ligase (paaK) CSB-YP305146ENV
CSB-EP305146ENV
CSB-BP305146ENV
CSB-MP305146ENV
CSB-EP305146ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized acetyltransferase ydcK (ydcK) CSB-YP305147ENV
CSB-EP305147ENV
CSB-BP305147ENV
CSB-MP305147ENV
CSB-EP305147ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative ABC transporter periplasmic-binding protein ydcS (ydcS) CSB-YP305148ENV
CSB-EP305148ENV
CSB-BP305148ENV
CSB-MP305148ENV
CSB-EP305148ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative transposase yncI (yncI) CSB-YP305149ENV
CSB-EP305149ENV
CSB-BP305149ENV
CSB-MP305149ENV
CSB-EP305149ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Autoinducer 2-binding protein lsrB (lsrB) CSB-YP305150ENV
CSB-EP305150ENV
CSB-BP305150ENV
CSB-MP305150ENV
CSB-EP305150ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable lysozyme from lambdoid prophage Qin (arrQ) CSB-YP305151ENV
CSB-EP305151ENV
CSB-BP305151ENV
CSB-MP305151ENV
CSB-EP305151ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative lambdoid prophage Qin defective integrase (intQ) CSB-YP305152ENV
CSB-EP305152ENV
CSB-BP305152ENV
CSB-MP305152ENV
CSB-EP305152ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane protein ydgK (ydgK), partial CSB-YP305153ENV1
CSB-EP305153ENV1
CSB-BP305153ENV1
CSB-MP305153ENV1
CSB-EP305153ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized oxidoreductase YdhV (ydhV) CSB-YP305154ENV
CSB-EP305154ENV
CSB-BP305154ENV
CSB-MP305154ENV
CSB-EP305154ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative cyclic di-GMP regulator CdgR (cdgR) CSB-YP305155ENV
CSB-BP305155ENV
CSB-MP305155ENV
CSB-EP305155ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli N-succinylarginine dihydrolase (astB) CSB-YP305156ENV
CSB-EP305156ENV
CSB-BP305156ENV
CSB-MP305156ENV
CSB-EP305156ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein ynjB (ynjB) CSB-YP305157ENV
CSB-EP305157ENV
CSB-BP305157ENV
CSB-MP305157ENV
CSB-EP305157ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yeaE (yeaE) CSB-YP305158ENV
CSB-EP305158ENV
CSB-BP305158ENV
CSB-MP305158ENV
CSB-EP305158ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yeaO (yeaO) CSB-YP305159ENV
CSB-EP305159ENV
CSB-BP305159ENV
CSB-MP305159ENV
CSB-EP305159ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YeaZ (yeaZ) CSB-YP305160ENV
CSB-EP305160ENV
CSB-BP305160ENV
CSB-MP305160ENV
CSB-EP305160ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yebT (yebT), partial CSB-YP305161ENV
CSB-EP305161ENV
CSB-BP305161ENV
CSB-MP305161ENV
CSB-EP305161ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yecT (yecT) CSB-YP305162ENV
CSB-EP305162ENV
CSB-BP305162ENV
CSB-MP305162ENV
CSB-EP305162ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized N-acetyltransferase YedL (yedL) CSB-YP305163ENV
CSB-EP305163ENV
CSB-BP305163ENV
CSB-MP305163ENV
CSB-EP305163ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative outer membrane protein YedS (yedS) CSB-YP305164ENV
CSB-EP305164ENV
CSB-BP305164ENV
CSB-MP305164ENV
CSB-EP305164ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein mtfA (mtfA) CSB-YP305165ENV
CSB-EP305165ENV
CSB-BP305165ENV
CSB-MP305165ENV
CSB-EP305165ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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