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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_312 (MPN_312) CSB-YP303428MLW
CSB-EP303428MLW
CSB-BP303428MLW
CSB-MP303428MLW
CSB-EP303428MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized RNA pseudouridine synthase MG209 homolog (MPN_292) CSB-YP303429MLW
CSB-EP303429MLW
CSB-BP303429MLW
CSB-MP303429MLW
CSB-EP303429MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae UPF0134 protein MPN_283 (MPN_283) CSB-YP303430MLW
CSB-EP303430MLW
CSB-BP303430MLW
CSB-MP303430MLW
CSB-EP303430MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG133 homolog (MPN_274), partial CSB-YP303431MLW1
CSB-EP303431MLW1
CSB-BP303431MLW1
CSB-MP303431MLW1
CSB-EP303431MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Tryptophan--tRNA ligase (trpS) CSB-YP303432MLW
CSB-EP303432MLW
CSB-BP303432MLW
CSB-MP303432MLW
CSB-EP303432MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG117 homolog (MPN_256) CSB-YP303433MLW
CSB-EP303433MLW
CSB-BP303433MLW
CSB-MP303433MLW
CSB-EP303433MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative protein phosphatase (MPN_247) CSB-YP303434MLW
CSB-EP303434MLW
CSB-BP303434MLW
CSB-MP303434MLW
CSB-EP303434MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized HTH-type transcriptional regulator MG101 homolog (MPN_239) CSB-YP303435MLW
CSB-EP303435MLW
CSB-BP303435MLW
CSB-MP303435MLW
CSB-EP303435MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MPN_234 (MPN_234) CSB-YP303436MLW
CSB-EP303436MLW
CSB-BP303436MLW
CSB-MP303436MLW
CSB-EP303436MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 30S ribosomal protein S6 (rpsF) CSB-YP303437MLW
CSB-EP303437MLW
CSB-BP303437MLW
CSB-MP303437MLW
CSB-EP303437MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae HPr kinase/phosphorylase (hprK) CSB-YP303438MLW
CSB-EP303438MLW
CSB-BP303438MLW
CSB-MP303438MLW
CSB-EP303438MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG076 homolog (MPN_214), partial CSB-YP303439MLW1
CSB-EP303439MLW1
CSB-BP303439MLW1
CSB-MP303439MLW1
CSB-EP303439MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP303440MLW
CSB-EP303440MLW
CSB-BP303440MLW
CSB-MP303440MLW
CSB-EP303440MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae 50S ribosomal protein L22 (rplV) CSB-YP303441MLW
CSB-EP303441MLW
CSB-BP303441MLW
CSB-MP303441MLW
CSB-EP303441MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized protein MG149.1 homolog (MPN_163), partial CSB-YP303442MLW1
CSB-EP303442MLW1
CSB-BP303442MLW1
CSB-MP303442MLW1
CSB-EP303442MLW1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Putative mgpC-like protein MPN_092 (MPN_092) CSB-YP303443MLW
CSB-EP303443MLW
CSB-BP303443MLW
CSB-MP303443MLW
CSB-EP303443MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Uncharacterized lipoprotein MPN_083 (MPN_083), partial CSB-YP303444MLW
CSB-BP303444MLW
CSB-MP303444MLW
CSB-EP303444MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yafS (yafS) CSB-YP303445ENV
CSB-EP303445ENV
CSB-BP303445ENV
CSB-MP303445ENV
CSB-EP303445ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative ferric transport system permease protein fbpB (fbpB), partial CSB-YP303446ENV1
CSB-EP303446ENV1
CSB-BP303446ENV1
CSB-MP303446ENV1
CSB-EP303446ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein sfmH (sfmH) CSB-YP303447ENV
CSB-EP303447ENV
CSB-BP303447ENV
CSB-MP303447ENV
CSB-EP303447ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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