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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neisseria meningitidis serogroup B Putative membrane protein insertion efficiency factor (NMB1906) CSB-YP303631NGG
CSB-EP303631NGG
CSB-BP303631NGG
CSB-MP303631NGG
CSB-EP303631NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor NrdR (nrdR) CSB-YP303632MVZ
CSB-EP303632MVZ
CSB-BP303632MVZ
CSB-MP303632MVZ
CSB-EP303632MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Transcriptional repressor NrdR (nrdR) CSB-YP303633SMU
CSB-EP303633SMU
CSB-BP303633SMU
CSB-MP303633SMU
CSB-EP303633SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0176 protein gbs1504 (gbs1504) CSB-YP303634SMH
CSB-EP303634SMH
CSB-BP303634SMH
CSB-MP303634SMH
CSB-EP303634SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0176 protein spyM18_0973 (spyM18_0973) CSB-YP303635SMU
CSB-EP303635SMU
CSB-BP303635SMU
CSB-MP303635SMU
CSB-EP303635SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0181 protein yoaH (yoaH) CSB-YP303636EOD
CSB-EP303636EOD
CSB-BP303636EOD
CSB-MP303636EOD
CSB-EP303636EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfoxide reductase catalytic subunit YedY (yedY) CSB-YP303637SWW
CSB-EP303637SWW
CSB-BP303637SWW
CSB-MP303637SWW
CSB-EP303637SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Listeria innocua serovar 6a UPF0223 protein lin1050 (lin1050) CSB-YP303638LGU
CSB-EP303638LGU
CSB-BP303638LGU
CSB-MP303638LGU
CSB-EP303638LGU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium UPF0227 protein ycfP (ycfP) CSB-YP303639SXB
CSB-EP303639SXB
CSB-BP303639SXB
CSB-MP303639SXB
CSB-EP303639SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant DegV domain-containing protein spyM18_1511 (spyM18_1511) CSB-YP303640SMU
CSB-EP303640SMU
CSB-BP303640SMU
CSB-MP303640SMU
CSB-EP303640SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UPF0234 protein Mb0581c (Mb0581c) CSB-YP303641MVH
CSB-EP303641MVH
CSB-BP303641MVH
CSB-MP303641MVH
CSB-EP303641MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Undecaprenyl-diphosphatase (uppP), partial CSB-YP303642SXB1
CSB-EP303642SXB1
CSB-BP303642SXB1
CSB-MP303642SXB1
CSB-EP303642SXB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Uracil phosphoribosyltransferase (upp) CSB-YP303643SUL
CSB-EP303643SUL
CSB-BP303643SUL
CSB-MP303643SUL
CSB-EP303643SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urease subunit beta (ureB) CSB-YP303644SKX
CSB-EP303644SKX
CSB-BP303644SKX
CSB-MP303644SKX
CSB-EP303644SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uridine kinase (udk) CSB-YP303645FMW
CSB-EP303645FMW
CSB-BP303645FMW
CSB-MP303645FMW
CSB-EP303645FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant UvrABC system protein B (uvrB), partial CSB-YP303646MVZ
CSB-EP303646MVZ
CSB-BP303646MVZ
CSB-MP303646MVZ
CSB-EP303646MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UvrABC system protein C (uvrC), partial CSB-YP303647SKX
CSB-EP303647SKX
CSB-BP303647SKX
CSB-MP303647SKX
CSB-EP303647SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv1556/MT1607 (Rv1556, MT1607) CSB-YP303648MVZ
CSB-EP303648MVZ
CSB-BP303648MVZ
CSB-MP303648MVZ
CSB-EP303648MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Uncharacterized HTH-type transcriptional regulator Rv3405c/MT3513 (Rv3405c, MT3513) CSB-YP303649MVZ
CSB-EP303649MVZ
CSB-BP303649MVZ
CSB-MP303649MVZ
CSB-EP303649MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Exodeoxyribonuclease 7 large subunit (xseA) CSB-YP303650SKX
CSB-EP303650SKX
CSB-BP303650SKX
CSB-MP303650SKX
CSB-EP303650SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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