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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Escherichia coli Lipoprotein bor homolog from lambdoid prophage DLP12 (borD) CSB-YP304377ENV
CSB-EP304377ENV
CSB-BP304377ENV
CSB-MP304377ENV
CSB-EP304377ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Periplasmic murein peptide-binding protein (mppA) CSB-YP304378ENV
CSB-BP304378ENV
CSB-MP304378ENV
CSB-EP304378ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Inner membrane transport protein ydhP (ydhP), partial CSB-YP304379ENV
CSB-EP304379ENV
CSB-BP304379ENV
CSB-MP304379ENV
CSB-EP304379ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ypdC (ypdC) CSB-YP304380ENV
CSB-EP304380ENV
CSB-BP304380ENV
CSB-MP304380ENV
CSB-EP304380ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydrogenase-4 component H (hyfH) CSB-YP304381ENV
CSB-EP304381ENV
CSB-BP304381ENV
CSB-MP304381ENV
CSB-EP304381ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Probable D,D-dipeptide transport system permease protein ddpC (ddpC), partial CSB-YP304382ENV1
CSB-EP304382ENV1
CSB-BP304382ENV1
CSB-MP304382ENV1
CSB-EP304382ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Sensor kinase CusS (cusS), partial CSB-YP304383ENV1
CSB-EP304383ENV1
CSB-BP304383ENV1
CSB-MP304383ENV1
CSB-EP304383ENV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized oxidoreductase YcjS (ycjS) CSB-YP304384ENV
CSB-EP304384ENV
CSB-BP304384ENV
CSB-MP304384ENV
CSB-EP304384ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative uncharacterized protein yddL (yddL) CSB-YP304385ENV
CSB-EP304385ENV
CSB-BP304385ENV
CSB-MP304385ENV
CSB-EP304385ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized symporter ydjN (ydjN), partial CSB-YP304386ENV
CSB-EP304386ENV
CSB-BP304386ENV
CSB-MP304386ENV
CSB-EP304386ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein yahJ (yahJ) CSB-YP304387ENV
CSB-EP304387ENV
CSB-BP304387ENV
CSB-MP304387ENV
CSB-EP304387ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein ydhW (ydhW) CSB-YP304388ENV
CSB-EP304388ENV
CSB-BP304388ENV
CSB-MP304388ENV
CSB-EP304388ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized protein YagF (yagF) CSB-YP304389ENV
CSB-EP304389ENV
CSB-BP304389ENV
CSB-MP304389ENV
CSB-EP304389ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Electron transport complex protein RnfC (rnfC) CSB-YP304390ENV
CSB-EP304390ENV
CSB-BP304390ENV
CSB-MP304390ENV
CSB-EP304390ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Phosphatase YfbT (yfbT) CSB-YP304391ENV
CSB-EP304391ENV
CSB-BP304391ENV
CSB-MP304391ENV
CSB-EP304391ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli 3-phenylpropionate/cinnamic acid dioxygenase ferredoxin--NAD (+) reductase component CSB-YP304392ENV
CSB-EP304392ENV
CSB-BP304392ENV
CSB-MP304392ENV
CSB-EP304392ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Protein gnsB (gnsB) CSB-YP304393ENV
CSB-EP304393ENV
CSB-BP304393ENV
CSB-MP304393ENV
CSB-EP304393ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Putative beta-xylosidase (yagH) CSB-YP304394ENV
CSB-EP304394ENV
CSB-BP304394ENV
CSB-MP304394ENV
CSB-EP304394ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ydcR (ydcR) CSB-YP304395ENV
CSB-EP304395ENV
CSB-BP304395ENV
CSB-MP304395ENV
CSB-EP304395ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Uncharacterized HTH-type transcriptional regulator ybdO (ybdO) CSB-YP304396ENV
CSB-EP304396ENV
CSB-BP304396ENV
CSB-MP304396ENV
CSB-EP304396ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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